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skos:definition |
MSH: A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166),CSP: experimental condition created by disrupting cells with homogenization, sonication, detergents, or other treatment, leaving intracellular components exposed to controlled manipulation of the medium.
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