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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
25
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pubmed:dateCreated |
1977-2-26
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pubmed:abstractText |
Zein messenger RNA was isolated from membrane-bound polyribosomes of developing maize kernels by oligo(dT)-cellulose chromatography. Translation of the mRNA in vitro yielded protein similar to native zein in amino acid content, ethanol solubility, and mobility on sodium dodecyl sulfate- polyacrylamide gels. The zein mRNA sedimented as a homogeneous peak on sucrose gradients and contained a poly(A)-rich region based upon hybridization to [3H]poly(U). The mRNA had an apparent molecular weight of 540 000 on agarose-acrylamide gels. It synthesized both 21 800 and 19 000 molecular weight zein components in the wheat-germ cell-free protein synthesis system. The possibility of a polycistronic mRNA or two mRNAs of similar molecular weight is considered.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5506-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:999825-Amino Acids,
pubmed-meshheading:999825-Cell-Free System,
pubmed-meshheading:999825-Molecular Weight,
pubmed-meshheading:999825-Nucleic Acid Hybridization,
pubmed-meshheading:999825-Poly A,
pubmed-meshheading:999825-Poly U,
pubmed-meshheading:999825-Polyribosomes,
pubmed-meshheading:999825-Protein Biosynthesis,
pubmed-meshheading:999825-RNA, Messenger,
pubmed-meshheading:999825-Triticum,
pubmed-meshheading:999825-Zea mays,
pubmed-meshheading:999825-Zein
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pubmed:year |
1976
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pubmed:articleTitle |
Isolation and in vitro translation of zein messenger ribonucleic acid.
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pubmed:publicationType |
Journal Article,
Comparative Study
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