Linked Life Data

9950100

Source:http://linkedlifedata.com/resource/pubmed/id/9950100

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-4-20
pubmed:abstractText
Adoptive immunotherapy using MHC-nonrestricted-lymphocytes, peripheral blood gammadelta T cells and NK cells was devised. Peripheral blood mononuclear cells (3 x 10(7)) were selected by immobilization to anti-CD3 monoclonal antibody for 4 days and cultured for 2 weeks in the presence of IL-2. Thereafter they were reactivated by 500 U/ml of IFN-alpha and 1000 U/ml of IL-2 for 1 hour. Enhancement of NK and LAK activities was confirmed. Peripheral blood gammadelta T cells proliferated in response to immobilized anti-CD3 antibody (3% to 30%). Approximately 6 x 10(9) BRM-activated killer (BAK) cells composed of CD56+ gammadelta T cells and CD56+ NK cells, were dispensed to cancer patients via intravenous drip infusion. Nine patients were treated with BAK cells every 2 weeks or every month on an outpatient basis. During the course of adoptive immunotherapy, the crossed affinity immunoelectrophoresis (CAIE) pattern of serum immunosuppressive acidic protein (IAP) was analysed. Both the production and glycosylation pattern of IAP is changed in response to tumor enlargement and may therefore act as a marker of the disease progression. During the course of BAK therapy, the glycosylation IAP pattern of 6 patients changed from tumor (T) to normal (N). In addition, the performance status of all patients was maintained at 90-100% of the Karnofsky scale and any side effects including fever were not observed during treatments with BAK cells. Moreover, the overall quality of life (QOL) of the patients, scored at the Face scale was favorable. In addition, blood levels of activated gammadelta T cells producing IFN-gamma were assayed as an indication marker of BAK therapy. The normal range of IFN-gamma producing gammadelta T cells comprised 6.9 +/- 0.9% of peripheral blood mononuclear cells (PBMC), according to a single cell FACScan analyses of PBMCs derived from normal individuals. IFN-gamma producing gammadelta T cells of Patients No. 8 and 9, who received extensive chemotherapy before initiation of BAK therapy, comprised only 0.2% and 2% of PBMC, respectively. These patients died 3 and 6 months after beginning BAK therapy. Peripheral blood gammadelta T cells of Patients Nos. 1-7 proliferated in response to immobilized anti-CD3 antibody and the frequency of IFN-gamma producing gammadelta T cells in PBMC preparation of these patients were over 3% before initiation of BAK therapy. Since our data show a positive correlation between survival time and initial gammadelta T cell counts, a low frequency of these cells may contraindicate BAK therapy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0921-299X
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
241-53
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9950100-Adult, pubmed-meshheading:9950100-Aged, pubmed-meshheading:9950100-Female, pubmed-meshheading:9950100-Humans, pubmed-meshheading:9950100-Immunologic Factors, pubmed-meshheading:9950100-Immunotherapy, Adoptive, pubmed-meshheading:9950100-Interferon-alpha, pubmed-meshheading:9950100-Interferon-gamma, pubmed-meshheading:9950100-Interleukin-2, pubmed-meshheading:9950100-Killer Cells, Lymphokine-Activated, pubmed-meshheading:9950100-Killer Cells, Natural, pubmed-meshheading:9950100-Lymphocyte Activation, pubmed-meshheading:9950100-Male, pubmed-meshheading:9950100-Middle Aged, pubmed-meshheading:9950100-Neoplasm Proteins, pubmed-meshheading:9950100-Neoplasms, pubmed-meshheading:9950100-Pilot Projects, pubmed-meshheading:9950100-Receptors, Antigen, T-Cell, gamma-delta, pubmed-meshheading:9950100-T-Lymphocyte Subsets, pubmed-meshheading:9950100-Tumor Markers, Biological
pubmed:year
1998
pubmed:articleTitle
The use of BRM-activated killer cells in adoptive immunotherapy: a pilot study with nine advanced cancer patients.
pubmed:affiliation
Division of Immunology, Research Institute, Miyagi Cancer Center, Natori, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't