Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-3-11
pubmed:abstractText
In the present study, we provide evidence that the alpha9 nicotinic acetylcholine receptor (nAChR) shares pharmacological properties with members of the Cys-loop family of receptors. Thus, the type A gamma-aminobutyric acid receptor antagonist bicuculline, the glycinergic antagonist strychnine, and the type 3 serotonin receptor antagonist ICS-205,930 block ACh-evoked currents in alpha9-injected Xenopus laevis oocytes with the following rank order of potency: strychnine > ICS-205,930 > bicuculline. Block by antagonists was reflected in an increase in the acetylcholine (ACh) EC50 value, with no changes in agonist maximal response or Hill coefficient, which suggests a competitive type of block. Moreover, whereas neither gamma-aminobutyric acid nor glycine modified ACh-evoked currents, serotonin blocked responses to ACh in a concentration-dependent manner. The present results suggest that the alpha9 nAChR must conserve in its primary structure some residues responsible for ligand binding common to other Cys-loop receptors. In addition, it adds further evidence that the alpha9 nAChR and the cholinergic receptor present at the base of cochlear outer hair cells have similar pharmacological properties.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1,2-bis(2-aminophenoxy)ethane-N,N,N'..., http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine, http://linkedlifedata.com/resource/pubmed/chemical/Barium, http://linkedlifedata.com/resource/pubmed/chemical/Bicuculline, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid, http://linkedlifedata.com/resource/pubmed/chemical/GABA Antagonists, http://linkedlifedata.com/resource/pubmed/chemical/Glycine, http://linkedlifedata.com/resource/pubmed/chemical/Glycine Agents, http://linkedlifedata.com/resource/pubmed/chemical/Indo-1 pentaacetoxymethyl ester, http://linkedlifedata.com/resource/pubmed/chemical/Indoles, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, GABA-A, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glycine, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nicotinic, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin, 5-HT3, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Serotonin, http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists, http://linkedlifedata.com/resource/pubmed/chemical/Strychnine, http://linkedlifedata.com/resource/pubmed/chemical/tropisetron
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
248-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9927615-Acetylcholine, pubmed-meshheading:9927615-Animals, pubmed-meshheading:9927615-Barium, pubmed-meshheading:9927615-Bicuculline, pubmed-meshheading:9927615-Calcium, pubmed-meshheading:9927615-Chelating Agents, pubmed-meshheading:9927615-DNA, Complementary, pubmed-meshheading:9927615-Egtazic Acid, pubmed-meshheading:9927615-Female, pubmed-meshheading:9927615-GABA Antagonists, pubmed-meshheading:9927615-Glycine, pubmed-meshheading:9927615-Glycine Agents, pubmed-meshheading:9927615-Indoles, pubmed-meshheading:9927615-Membrane Potentials, pubmed-meshheading:9927615-Oocytes, pubmed-meshheading:9927615-Receptors, GABA-A, pubmed-meshheading:9927615-Receptors, Glycine, pubmed-meshheading:9927615-Receptors, Nicotinic, pubmed-meshheading:9927615-Receptors, Serotonin, pubmed-meshheading:9927615-Receptors, Serotonin, 5-HT3, pubmed-meshheading:9927615-Recombinant Fusion Proteins, pubmed-meshheading:9927615-Serotonin, pubmed-meshheading:9927615-Serotonin Antagonists, pubmed-meshheading:9927615-Strychnine, pubmed-meshheading:9927615-Xenopus laevis
pubmed:year
1999
pubmed:articleTitle
The alpha9 nicotinic acetylcholine receptor shares pharmacological properties with type A gamma-aminobutyric acid, glycine, and type 3 serotonin receptors.
pubmed:affiliation
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas-Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't