Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1999-2-18
|
| pubmed:abstractText |
It has been reported that reactivation of human herpesvirus-6 (HHV-6) causes a failure of hematopoiesis. To clarify the mechanisms of bone marrow suppression induced by HHV-6 infection, it is necessary to establish an in vitro model of HHV-6 infection in hematopoietic progenitor cells. We have established two novel Philadelphia chromosome-positive myeloid cell lines, SAS413 and SAS527, which possess different hematologic characteristics and show distinct susceptibility to infection by HHV-6, from a patient with blast crisis of chronic myelogenous leukemia (CML). HHV-6 subgroup A (HHV-6A) showed marked replication in SAS413, forming syncytia and inducing cell lysis in short-term culture. On the other hand, HHV-6A-inoculated SAS527 continued to proliferate without cell lysis and only a few cells showed HHV-6 antigen expression. In contrast to HHV-6A infection, inoculation with HHV-6 subgroup B (HHV-6B) did not induce any cytopathic effect (CPE) or viral antigen expression in either of the cell lines. Although HHV-6B replication was undetectable, the presence of the HHV-6 genome in both cell lines was shown by polymerase chain reaction (PCR) during culture for more than 10 months, suggesting that HHV-6B latently infected SAS413 and SAS527. Phorbol ester treatment of SAS527 latently infected with HHV-6B resulted in reactivation of HHV-6, as shown by the appearance of a CPE, positive reactivity for the HHV-6 antigen, and isolation of infectious HHV-6. These novel cell lines should be useful for studying the mechanisms of HHV-6-induced hematopoietic failure and HHV-6 latency and reactivation, as well as differentiation, of the myeloid cell lineage.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
93
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
991-9
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9920848-Adult,
pubmed-meshheading:9920848-Antigens, Viral,
pubmed-meshheading:9920848-Blast Crisis,
pubmed-meshheading:9920848-Cytopathogenic Effect, Viral,
pubmed-meshheading:9920848-DNA, Viral,
pubmed-meshheading:9920848-Hematopoietic Stem Cells,
pubmed-meshheading:9920848-Herpesvirus 6, Human,
pubmed-meshheading:9920848-Humans,
pubmed-meshheading:9920848-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:9920848-Male,
pubmed-meshheading:9920848-Neoplastic Stem Cells,
pubmed-meshheading:9920848-Polymerase Chain Reaction,
pubmed-meshheading:9920848-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9920848-Tumor Cells, Cultured,
pubmed-meshheading:9920848-Virus Activation,
pubmed-meshheading:9920848-Virus Latency,
pubmed-meshheading:9920848-Virus Replication
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Latent infection and reactivation of human herpesvirus 6 in two novel myeloid cell lines.
|
| pubmed:affiliation |
First Department of Internal Medicine, Ehime University School of Medicine, Ehime, Japan. yasukawa@m.ehimeu.ac.jp
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|