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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-2-4
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pubmed:abstractText |
Typing methods utilising DNA technology were applied to a collection of Trichophyton mentagrophytes and T. rubrum isolates from skin and nail infections. The methods included restriction enzyme analysis (REA), hybridisation with the DNA probe poly (dG-dT), randomly amplified polymorphic DNA (RAPD) by PCR and restriction analysis of a segment of PCR-amplified rDNA. All these tests successfully differentiated the species, but few intra-species differences were detected. REA demonstrated some isolate variation, but this was limited and difficult to interpret, making it unsuitable as a typing tool. RAPD demonstrated few variations amongst T. mentagrophytes and none in T. rubrum.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-2615
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9920123-DNA, Fungal,
pubmed-meshheading:9920123-DNA, Ribosomal,
pubmed-meshheading:9920123-DNA Probes,
pubmed-meshheading:9920123-Genetic Techniques,
pubmed-meshheading:9920123-Humans,
pubmed-meshheading:9920123-Molecular Probe Techniques,
pubmed-meshheading:9920123-Mycological Typing Techniques,
pubmed-meshheading:9920123-Nails,
pubmed-meshheading:9920123-Nucleic Acid Hybridization,
pubmed-meshheading:9920123-Onychomycosis,
pubmed-meshheading:9920123-Polydeoxyribonucleotides,
pubmed-meshheading:9920123-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:9920123-Random Amplified Polymorphic DNA Technique,
pubmed-meshheading:9920123-Restriction Mapping,
pubmed-meshheading:9920123-Sensitivity and Specificity,
pubmed-meshheading:9920123-Skin,
pubmed-meshheading:9920123-Tinea,
pubmed-meshheading:9920123-Trichophyton
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pubmed:year |
1999
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pubmed:articleTitle |
Application of molecular typing methods to dermatophyte species that cause skin and nail infections.
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pubmed:affiliation |
Department of Microbial Diseases, St John's Institute of Dermatology, St Thomas' Hospital, UMDS, London.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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