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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9-10
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pubmed:dateCreated |
1999-4-2
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pubmed:abstractText |
The mouse Mx1 gene encodes an interferon (IFN)-inducible nuclear protein and confers resistance to influenza virus infection. The standard laboratory mouse strains all carry the Mx1- allele and are susceptible to influenza virus. In this study, several mouse strains established from wild mice were tested to determine their Mx1+ or Mx1- allele status with polymerase chain reaction-restriction fragment length variation (PCR-RFLV), sequence analysis, reverse transcription (RT)-PCR, and immunofluorescence staining. All of the mouse strains originating from wild mice were found uniformly to carry the Mx1+ allele. Therefore, it is conceivable that the Mx1+ allele in wild populations serves a function against some pathogens related to orthomyxoviruses. The PCR-RFLV and sequence analysis allowed us to classify the Mx1+ alleles of the laboratory and wild-origin mouse strains into distinct classes. RT-PCR and immunofluorescence staining demonstrated that the Mx1 transcripts and proteins were induced by IFN-alpha/beta in macrophages from wild mouse species.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-2928
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
311-22
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pubmed:dateRevised |
2005-5-27
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pubmed:meshHeading |
pubmed-meshheading:9919357-Animals,
pubmed-meshheading:9919357-Base Sequence,
pubmed-meshheading:9919357-Blotting, Southern,
pubmed-meshheading:9919357-Cell Nucleus,
pubmed-meshheading:9919357-Fluorescent Antibody Technique,
pubmed-meshheading:9919357-GTP-Binding Proteins,
pubmed-meshheading:9919357-Interferon-alpha,
pubmed-meshheading:9919357-Interferon-beta,
pubmed-meshheading:9919357-Macrophages, Peritoneal,
pubmed-meshheading:9919357-Mice,
pubmed-meshheading:9919357-Mice, Inbred BALB C,
pubmed-meshheading:9919357-Mice, Inbred C57BL,
pubmed-meshheading:9919357-Mice, Inbred CBA,
pubmed-meshheading:9919357-Mice, Inbred Strains,
pubmed-meshheading:9919357-Molecular Sequence Data,
pubmed-meshheading:9919357-Orthomyxoviridae,
pubmed-meshheading:9919357-Point Mutation,
pubmed-meshheading:9919357-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:9919357-Proteins,
pubmed-meshheading:9919357-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9919357-Sequence Homology, Nucleic Acid
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pubmed:year |
1998
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pubmed:articleTitle |
Characterization and expression of the Mx1 gene in wild mouse species.
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pubmed:affiliation |
Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
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pubmed:publicationType |
Journal Article
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