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rdf:type |
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lifeskim:mentions |
umls-concept:C0001483,
umls-concept:C0169665,
umls-concept:C0205263,
umls-concept:C0332281,
umls-concept:C0919418,
umls-concept:C1152568,
umls-concept:C1155873,
umls-concept:C1332737,
umls-concept:C1332739,
umls-concept:C1511576,
umls-concept:C1705523
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pubmed:issue |
3
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pubmed:dateCreated |
1999-2-9
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pubmed:abstractText |
A recombinant adenovirus containing the Von Hippel-Lindau (VHL) cDNA was constructed (AdVHL) and used to investigate the function of this tumor suppressor gene. Exposure of renal and breast cancer cell lines to AdVHL resulted in high levels of VHL mRNA and protein. AdVHL infection resulted in G1 cell cycle arrest and growth inhibition of renal and breast cancer cell lines. AdVHL-mediated cell cycle arrest was associated with induction of the cyclin-dependent kinase inhibitor (CDKI) p27Kip1 and inhibition of CDK2 and cyclinB1-dependent cdc2 activities. Nuclear run-on analyses and actinomycin D inhibition studies indicate that the induction of p27Kip1 RNA by VHL is mediated at least in part through an increase in p27Kip1 mRNA synthesis. Furthermore, [35S]methionine pulse-chase studies indicate that the increase in p27Kip expression is also regulated through posttranscriptional control mechanisms. These studies support a novel concept that the tumor suppressor gene VHL controls cell cycle progression by regulation of p27Kip1 at both the mRNA and protein levels.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/VHL protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Von Hippel-Lindau Tumor Suppressor...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
253
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
672-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9918786-Adenoviridae,
pubmed-meshheading:9918786-Cell Cycle,
pubmed-meshheading:9918786-Cell Cycle Proteins,
pubmed-meshheading:9918786-Cyclin-Dependent Kinase Inhibitor p27,
pubmed-meshheading:9918786-Cyclin-Dependent Kinases,
pubmed-meshheading:9918786-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9918786-Genes, Tumor Suppressor,
pubmed-meshheading:9918786-Humans,
pubmed-meshheading:9918786-Ligases,
pubmed-meshheading:9918786-Microtubule-Associated Proteins,
pubmed-meshheading:9918786-Protein Biosynthesis,
pubmed-meshheading:9918786-Proteins,
pubmed-meshheading:9918786-RNA, Messenger,
pubmed-meshheading:9918786-RNA, Neoplasm,
pubmed-meshheading:9918786-Recombinant Proteins,
pubmed-meshheading:9918786-Tumor Cells, Cultured,
pubmed-meshheading:9918786-Tumor Suppressor Proteins,
pubmed-meshheading:9918786-Ubiquitin-Protein Ligases,
pubmed-meshheading:9918786-Von Hippel-Lindau Tumor Suppressor Protein
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pubmed:year |
1998
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pubmed:articleTitle |
Recombinant adenovirus expressing Von Hippel-Lindau-mediated cell cycle arrest is associated with the induction of cyclin-dependent kinase inhibitor p27Kip1.
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pubmed:affiliation |
Medicine Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
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pubmed:publicationType |
Journal Article
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