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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1 Suppl
|
| pubmed:dateCreated |
1999-1-27
|
| pubmed:abstractText |
cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
1061-4036
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
10-4
|
| pubmed:dateRevised |
2005-11-16
|
| pubmed:meshHeading |
pubmed-meshheading:9915494-Animals,
pubmed-meshheading:9915494-DNA, Complementary,
pubmed-meshheading:9915494-Databases, Factual,
pubmed-meshheading:9915494-Expressed Sequence Tags,
pubmed-meshheading:9915494-Gene Expression,
pubmed-meshheading:9915494-Humans,
pubmed-meshheading:9915494-Image Processing, Computer-Assisted,
pubmed-meshheading:9915494-Molecular Probe Techniques,
pubmed-meshheading:9915494-Oligonucleotide Array Sequence Analysis
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Expression profiling using cDNA microarrays.
|
| pubmed:affiliation |
Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
|
| pubmed:publicationType |
Journal Article,
Review
|