Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1978-10-27
pubmed:abstractText
Bacillus megaterium N.C.T.C. no. 10342 exhibits glutamate synthetase (EC 2.6.1.53) and glutamate dehydrogenase (EC 1.4.1.4) activities. Concentrations of glutamate synthase were high when the bacteria were grown on 3mM-NH4Cl and low when they were grown on 100mM-NH4Cl, whereas glutamate dehydrogenase concentrations were higher when the bacteria were grown on 100mM-NH4Cl than on 3mM-NH4Cl. Glutamate synthase and glutamate dehydrogenase were purified to homogeneity from B. megaterium grown in 10mM-glucose/10mM-NH4Cl. The purified enzymes had mol.wts. 840000 and 270000 for glutamate synthase and glutamate dehydrogenase respectively. The Km values for substrates with NADPH and coenzyme were (glutamate synthase activity shown first) 9 micron and 360 micron for 2-oxoglutarate, 7.1 micron and 8.7 micron for NADPH, and 0.2 mM for glutamine and 22 mM for NH4Cl, similar values to those of enzymes from Escherichia coli. Glutamate synthase contained NH3-dependent activity (different from authentic glutamate dehydrogenase), which was enhanced 4-fold during treatment at pH 4.6 NH3-dependent activity was generally about 2% of the glutamine-dependent activity. Amidination of glutamate synthase by the bi-functional cross-linking reagent dimethyl suberimidate inactivated glutamine-dependent glutamate synthase activity, but increased NH3-dependent activity. A cross-linked structure of mol.wt. approx 200000 was the main product formed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-13563458, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-13767412, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-16906, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-235298, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-238942, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-241744, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4144743, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4321766, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4338318, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4344229, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4364420, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4396790, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4565085, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4861258, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4913206, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4944634, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4993401, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-4995907, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-5038877, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-5420057, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-5806584, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-6449, http://linkedlifedata.com/resource/pubmed/commentcorrection/99144-6450
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
173
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-52
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Purification and properties of glutamate synthase and glutamate dehydrogenase from Bacillus megaterium.
pubmed:publicationType
Journal Article