|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5398
|
|
pubmed:dateCreated |
1999-1-21
|
|
pubmed:abstractText |
Stable delivery of a therapeutic protein under pharmacologic control was achieved through in vivo somatic gene transfer. This system was based on the expression of two chimeric, human-derived proteins that were reconstituted by rapamycin into a transcription factor complex. A mixture of two adeno-associated virus vectors, one expressing the transcription factor chimeras and one containing erythropoietin (Epo) under the control of a promoter responsive to the transcription factor, was injected into skeletal muscle of immune-competent mice. Administration of rapamycin resulted in 200-fold induction of plasma Epo. Stable engraftment of this humanized system in immune-competent mice was achieved for 6 months with similar results for at least 3 months in a rhesus monkey.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0036-8075
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
283
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
88-91
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:9872748-Animals,
pubmed-meshheading:9872748-Cytomegalovirus,
pubmed-meshheading:9872748-Dependovirus,
pubmed-meshheading:9872748-Erythropoietin,
pubmed-meshheading:9872748-Female,
pubmed-meshheading:9872748-Gene Expression Regulation,
pubmed-meshheading:9872748-Gene Therapy,
pubmed-meshheading:9872748-Gene Transfer Techniques,
pubmed-meshheading:9872748-Genetic Vectors,
pubmed-meshheading:9872748-Hematocrit,
pubmed-meshheading:9872748-Injections, Intramuscular,
pubmed-meshheading:9872748-Macaca mulatta,
pubmed-meshheading:9872748-Male,
pubmed-meshheading:9872748-Mice,
pubmed-meshheading:9872748-Mice, Inbred BALB C,
pubmed-meshheading:9872748-Mice, Nude,
pubmed-meshheading:9872748-Muscle, Skeletal,
pubmed-meshheading:9872748-Promoter Regions, Genetic,
pubmed-meshheading:9872748-Recombinant Fusion Proteins,
pubmed-meshheading:9872748-Recombinant Proteins,
pubmed-meshheading:9872748-Sirolimus,
pubmed-meshheading:9872748-Transcription Factors
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Regulated delivery of therapeutic proteins after in vivo somatic cell gene transfer.
|
|
pubmed:affiliation |
Institute for Human Gene Therapy, University of Pennsylvania, Philadelphia, PA 19104, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
