Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1999-3-10
|
| pubmed:abstractText |
We analyzed an X-linked metallothionein-vasopressin (MTVP) fusion transgene that undergoes X-chromosome inactivation (X inactivation) and an X-linked transferrin (TFN) transgene that escapes X inactivation with respect to methylation in the 5' regulatory regions. The MTVP transgene promoter region is unmethylated when the transgene is on the active X chromosome and methylated when on the inactive X chromosome. Interestingly, the MTVP transgene is not detectably transcribed from the male X chromosome, although it is unmethylated, consistent with its availability for transcription. The TFN transgene promoter region is hypomethylated on both the active and inactive X chromosomes, consistent with its expression from both chromosomes. The TFN and MTVP transgenes have been mapped to chromosomal regions D and C, respectively, by fluorescence in situ hybridization. These observations are discussed in the context of our understanding of the role of DNA methylation in the spread and maintenance of X-chromosome inactivation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0967-3849
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
397-404
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9872669-Animals,
pubmed-meshheading:9872669-Artificial Gene Fusion,
pubmed-meshheading:9872669-Blotting, Northern,
pubmed-meshheading:9872669-Chromosome Mapping,
pubmed-meshheading:9872669-DNA Methylation,
pubmed-meshheading:9872669-DNA Probes,
pubmed-meshheading:9872669-Dosage Compensation, Genetic,
pubmed-meshheading:9872669-Female,
pubmed-meshheading:9872669-Genomic Imprinting,
pubmed-meshheading:9872669-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9872669-Male,
pubmed-meshheading:9872669-Metallothionein,
pubmed-meshheading:9872669-Mice,
pubmed-meshheading:9872669-Mice, Inbred C57BL,
pubmed-meshheading:9872669-Promoter Regions, Genetic,
pubmed-meshheading:9872669-Transferrin,
pubmed-meshheading:9872669-Transgenes,
pubmed-meshheading:9872669-Vasopressins
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Comparative methylation analysis of murine transgenes that undergo or escape X-chromosome inactivation.
|
| pubmed:affiliation |
Department of Biology, San Francisco State University, CA 94132-1722, USA. goldman@sfsu.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|