Linked Life Data

9869416

Source:http://linkedlifedata.com/resource/pubmed/id/9869416

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-1-15
pubmed:databankReference
pubmed:abstractText
A cDNA of tobacco BY-2 cells corresponding to an mRNA species which was rapidly induced by methyl jasmonate (MeJA) in the presence of cycloheximide (CHX) was found to encode ornithine decarboxylase (ODC). Another cDNA from a MeJA-inducible mRNA encoded S-adenosylmethionine synthase (SAMS). Although these enzymes could be involved in the biosynthesis of polyamines, the level of putrescine, a reaction product of ODC, increased slowly and while the levels of spermidine and spermine did not change following treatment of cells with MeJA. However, N-methylputrescine, which is a precursor of pyrrolidine ring of nicotine, started to increase shortly after MeJA-treatment of cells and the production of nicotine occured thereafter. The levels of mRNA for arginine decarboxylase (ADC), an alternative enzyme for putrescine synthesis, and that for S-adenosylmethionine decarboxylase (SAMDC), required for polyamine synthesis, were not affected by MeJA. In addition to mRNAs for ODC and SAMS, mRNA for putrescine N-methyltransferase (PMT) was also induced by MeJA. Unlike the MeJA-induction of ODC mRNA, MeJA-induction of SAMS and PMT mRNAs were blocked by CHX. The level of ODC mRNA declined after 1 to 4 h following MeJA treatment, while the levels of mRNAs for SAMS and PMT continued to increase. Auxin significantly reduced the MeJA-inducible accumulation of mRNAs for ODC, SAMS and PMT. These results indicate that MeJA sequentially induces expression of a series of genes involved in nicotine biosynthesis by multiple regulatory mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1101-11
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9869416-Acetic Acids, pubmed-meshheading:9869416-Amino Acid Sequence, pubmed-meshheading:9869416-Base Sequence, pubmed-meshheading:9869416-Cells, Cultured, pubmed-meshheading:9869416-Cyclopentanes, pubmed-meshheading:9869416-DNA, Complementary, pubmed-meshheading:9869416-DNA Primers, pubmed-meshheading:9869416-Gene Expression Regulation, Enzymologic, pubmed-meshheading:9869416-Gene Expression Regulation, Plant, pubmed-meshheading:9869416-Molecular Sequence Data, pubmed-meshheading:9869416-Nicotine, pubmed-meshheading:9869416-Ornithine Decarboxylase, pubmed-meshheading:9869416-Oxylipins, pubmed-meshheading:9869416-Plant Growth Regulators, pubmed-meshheading:9869416-Plants, Toxic, pubmed-meshheading:9869416-RNA, Messenger, pubmed-meshheading:9869416-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:9869416-Sequence Alignment, pubmed-meshheading:9869416-Sequence Homology, Amino Acid, pubmed-meshheading:9869416-Tobacco, pubmed-meshheading:9869416-Transcription, Genetic
pubmed:year
1998
pubmed:articleTitle
Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosynthesis in tobacco cell cultures.
pubmed:affiliation
Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences Nagoya University, Chikusa, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't