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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1999-2-25
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pubmed:abstractText |
We determined the spatial and temporal dynamics of the increase in intracellular Ca2+ levels [Ca2+]i produced by substance P (SP) in dorsal horn neurons. A microinjection technique was used to apply minute amounts of SP to small areas of cultured neurons loaded with the Ca2+ indicator fura-2. Five successive applications of SP to the soma produced short-lasting (< 50 s) increases in [Ca2+]i that became gradually smaller, indicating receptor desensitization. Focal application of SP to a distal locus in a neurite produced a brief (12 s) increase in [Ca2+]i that travelled down the dendrite but did not spread into cell soma. Prolonged application of SP to these neurons caused the appearance of varicosities in their dendrites.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0959-4965
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3369-74
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9855282-Animals,
pubmed-meshheading:9855282-Calcium,
pubmed-meshheading:9855282-Cells, Cultured,
pubmed-meshheading:9855282-Dendrites,
pubmed-meshheading:9855282-Fluorescent Dyes,
pubmed-meshheading:9855282-Fura-2,
pubmed-meshheading:9855282-Glutamic Acid,
pubmed-meshheading:9855282-Microscopy, Fluorescence,
pubmed-meshheading:9855282-Neurons,
pubmed-meshheading:9855282-Pain,
pubmed-meshheading:9855282-Receptors, Neurokinin-1,
pubmed-meshheading:9855282-Spinal Cord,
pubmed-meshheading:9855282-Substance P
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pubmed:year |
1998
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pubmed:articleTitle |
Substance P induces brief, localized increase in [Ca2+]i in dorsal horn neurons.
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pubmed:affiliation |
Digestive Diseases Research Center, Department of Medicine, University of California, Los Angeles 90095, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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