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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
13
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pubmed:dateCreated |
1999-2-26
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pubmed:abstractText |
We describe a genome-wide functional assay for rapid isolation of cell clones and genetic elements responsive to specific stimuli. A promoterless beta-lactamase reporter gene was transfected into a human T-cell line to generate a living library of reporter-tagged clones. When loaded with a cell-permeable fluorogenic substrate, the cell library simultaneously reports the expression of a large number of endogenous genes. Flow cytometry was used to recover individual clones whose reporter-tagged genes were either induced or repressed following T-cell activation. Responsive clones were expanded and analyzed pharmacologically to identify patterns of regulation associated with specific genes. Although demonstrated using T cells, the genomic assay could be applied to map downstream transcriptional consequences for any propagating cell line in response to any stimulus of interest.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
|
pubmed:issn |
1087-0156
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1329-33
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:9853613-Cell Separation,
pubmed-meshheading:9853613-Enzyme Activation,
pubmed-meshheading:9853613-Flow Cytometry,
pubmed-meshheading:9853613-Fluorescent Dyes,
pubmed-meshheading:9853613-Genes, Reporter,
pubmed-meshheading:9853613-Genome,
pubmed-meshheading:9853613-Humans,
pubmed-meshheading:9853613-Jurkat Cells,
pubmed-meshheading:9853613-Signal Transduction,
pubmed-meshheading:9853613-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9853613-Thapsigargin,
pubmed-meshheading:9853613-beta-Lactamases
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pubmed:year |
1998
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pubmed:articleTitle |
A genome-wide functional assay of signal transduction in living mammalian cells.
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pubmed:affiliation |
Aurora Biosciences Corporation, San Diego, CA 92121, USA.
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pubmed:publicationType |
Journal Article
|