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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1998-12-22
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pubmed:abstractText |
The authors relate the realization, evaluation and eco-epidemiological applications of a "field-test": the agglutination of latex particles coated with a soluble antigen of Leishmania infantum in the presence of homologous antibodies. Evaluated on 1,035 canine sera, the sensitivity of the latex agglutination test (LAT) was 93.4% compared to the indirect fluorescent antibody test (IFAT). 90 node cultures were carried out on dogs with positive or negative LAT and/or positive or negative IFAT. The frequency of positive node cultures (70%) as versus positive LAT came between the results obtained for an IFAT > 1/40 (64%) and IFAT > 1/80 (73%). 32/33 (97%) dogs had positive node culture, LAT and IFIAT(> 1/80). 6 dogs had negative LAT but positive node culture: 5 of these had also an IFAT < 1/160. This test was used in the field on several eco-epidemiological surveys in leishmanian enzootic areas. Node cultures were made on the dogs with positive TL. 39 strains were isolated: 18 in Algeria, 15 in Morocco, 2 in Syria and 4 in Yemen. 13/39 strains were obtained from dogs with IFI < 1/160: 2 at 1/20, 8 at 1/40 and 3 at 1/80. In Algeria this test was also used for the diagnosis of human visceral leishmaniasis in a child. This quick, simple, sensitive and specific test could be usefully carried out on "field" surveys for the diagnosis of visceral-leishmaniasis in animals and human beings.
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pubmed:language |
fre
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0037-9085
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
300-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9846221-Animals,
pubmed-meshheading:9846221-Antibodies, Protozoan,
pubmed-meshheading:9846221-Dog Diseases,
pubmed-meshheading:9846221-Dogs,
pubmed-meshheading:9846221-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:9846221-Humans,
pubmed-meshheading:9846221-Latex Fixation Tests,
pubmed-meshheading:9846221-Leishmania infantum,
pubmed-meshheading:9846221-Leishmaniasis, Visceral,
pubmed-meshheading:9846221-Lymph Nodes,
pubmed-meshheading:9846221-Sensitivity and Specificity
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pubmed:year |
1998
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pubmed:articleTitle |
[Canine leishmaniasis from Leishmania infantum: value and production of the latex test. Ecoepidemiologic applications].
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pubmed:affiliation |
Laboratoire d'écologie médicale et de pathologie parasitaire, Faculté de médecine, Montpellier, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract
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