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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1998-12-10
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pubmed:abstractText |
Erythroid and megakaryocyte lineages are closely linked and may share a common bipotent progenitor. However, the mechanisms associated with cell lineage commitment are not fully understood. The K562 erythroleukemia cell line serves as a model to study the biochemical changes associated with erythroid and megakaryocyte (E/M) differentiation. We have previously established that PMA-induced megakaryocyte differentiation of K562 cells requires the activity of the MEK/MAPK pathway (Herrera et al Exp Cell Res 1998; 238: 407-414). Here, we show that the PMA-induced phenotypic changes of K562 cells such as polylobulation of the nucleus and Pyk2 expression are independent of MAPK activation. In addition, we also demonstrate that inhibition of the basal activity of the extracellular regulated kinase (ERK/MAPK) pathway enhances the erythroid phenotype of these cells. These results suggest that the MAPK pathway regulates the E/M lineage commitment of K562 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/MAP2K1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/MAP2K2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0887-6924
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1951-61
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9844925-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:9844925-Carcinogens,
pubmed-meshheading:9844925-Cell Differentiation,
pubmed-meshheading:9844925-Enzyme Activation,
pubmed-meshheading:9844925-Erythroid Precursor Cells,
pubmed-meshheading:9844925-Focal Adhesion Kinase 2,
pubmed-meshheading:9844925-Humans,
pubmed-meshheading:9844925-K562 Cells,
pubmed-meshheading:9844925-MAP Kinase Kinase 1,
pubmed-meshheading:9844925-MAP Kinase Kinase 2,
pubmed-meshheading:9844925-Megakaryocytes,
pubmed-meshheading:9844925-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:9844925-Phenotype,
pubmed-meshheading:9844925-Phosphorylation,
pubmed-meshheading:9844925-Protein-Serine-Threonine Kinases,
pubmed-meshheading:9844925-Protein-Tyrosine Kinases,
pubmed-meshheading:9844925-Signal Transduction,
pubmed-meshheading:9844925-Tetradecanoylphorbol Acetate
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pubmed:year |
1998
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pubmed:articleTitle |
PMA-induced phenotypic changes in K562 cells: MAPK-dependent and -independent events.
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pubmed:affiliation |
Department of Cell Biology, Parke-Davis Pharmaceutical Research Division of Warner-Lambert Company, Ann Arbor, MI 48105, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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