Linked Life Data

9843431

Source:http://linkedlifedata.com/resource/pubmed/id/9843431

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
47
pubmed:dateCreated
1998-12-31
pubmed:databankReference
pubmed:abstractText
The role of alpha-helix E' of the RNase H domain of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) in template-primer binding and fidelity of DNA synthesis was investigated by using a series of mutant enzymes with deletions of 4, 8, 12, 16, and 20 amino acids at the C-terminal end of the 66 kDa subunit. The dissociation equilibrium constants (Kd) of wild-type HIV-1 RT and 38/16mer and 47/25mer DNA/DNA template-primer complexes were 2.2 +/- 0.7 and 0.69 +/- 0.35 nM, respectively. Deletions involving partial or total removal of alpha-helix E' rendered enzymes with a 2-5-fold decrease in binding affinity. Misinsertion and mispair extension fidelity of DNA synthesis of the wild-type enzyme and truncated mutants were determined by using both DNA/DNA template-primers and a 47/25mer RNA/DNA complex. In all cases, incorporation assays were done in the same sequence context, which was taken from the viral gag gene. The removal of alpha-helix E' had little effect on fidelity as determined with the three template-primers. Misinsertion fidelity assays showed that the specificity of mismatch formation was A:C approximately A:G > A:A for the DNA template and A:C > A:G approximately A:A for the RNA template, in 47/25mers. The specificity of extending mispaired 3'-termini was similar with both 47/25mers: A:C > A:A approximately A:G. However, the efficiency of transversion mispair extension was higher with RNA templates. The results reported in this paper suggest that alpha-helix E' may stabilize the RT/template-primer interaction, but does not have a strong influence in the correct positioning of the template-primer at the polymerase active site.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16636-44
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Studies on the effects of truncating alpha-helix E' of p66 human immunodeficiency virus type 1 reverse transcriptase on template-primer binding and fidelity of DNA synthesis.
pubmed:affiliation
Centro de Biología Molecular "Severo Ochoa", CSIC-Universidad Autónoma de Madrid, Spain. lmenedez@cbm.uam.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't