|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
1998-12-16
|
|
pubmed:abstractText |
Opposing cells often communicate signalling events using multivalent interactions between receptors present on their cell surface. For example, T cells are typically activated when the T cell receptor (TCR) and its associated costimulatory molecules are multivalently engaged by the appropriate ligands present on an antigen presenting cell. In this report, yeast expressing high cell-surface levels of a TCR ligand (a recombinant antibody to the TCR Vbeta domain) were shown to act as 'pseudo' antigen presenting cells and induce T cell activation as monitored by increased levels of CD25 and CD69 and by downregulation of cell surface TCR. Similar levels of T cell activation could occur even when a 30-fold excess of irrelevant yeast was present, suggesting that such a yeast display system, by virtue of its ability to present ligands multivalently, may be used in highly sensitive procedures to identify novel polypeptides that interact multivalently with cell surface receptors and thereby trigger specific cellular responses.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD69 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Latex,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-CD3 Complex, Antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/immunoglobulin Fv
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0022-1759
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
220
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
179-88
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9839939-Animals,
pubmed-meshheading:9839939-Antigen Presentation,
pubmed-meshheading:9839939-Antigens, CD,
pubmed-meshheading:9839939-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:9839939-Down-Regulation,
pubmed-meshheading:9839939-Flow Cytometry,
pubmed-meshheading:9839939-Hybridomas,
pubmed-meshheading:9839939-Immunoglobulin Fragments,
pubmed-meshheading:9839939-Immunoglobulin Variable Region,
pubmed-meshheading:9839939-Latex,
pubmed-meshheading:9839939-Lectins, C-Type,
pubmed-meshheading:9839939-Ligands,
pubmed-meshheading:9839939-Lymphocyte Activation,
pubmed-meshheading:9839939-Mice,
pubmed-meshheading:9839939-Mice, Transgenic,
pubmed-meshheading:9839939-Microspheres,
pubmed-meshheading:9839939-Peptide Fragments,
pubmed-meshheading:9839939-Receptor-CD3 Complex, Antigen, T-Cell,
pubmed-meshheading:9839939-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:9839939-Receptors, Interleukin-2,
pubmed-meshheading:9839939-Recombinant Fusion Proteins,
pubmed-meshheading:9839939-Saccharomyces cerevisiae,
pubmed-meshheading:9839939-Spleen,
pubmed-meshheading:9839939-Transfection
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
A yeast surface display system for the discovery of ligands that trigger cell activation.
|
|
pubmed:affiliation |
Department of Biochemistry, University of Illinois, Urbana 61801, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
