Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
1999-2-10
|
pubmed:abstractText |
The purpose of this study was to evaluate whether serological assays and immunohistochemical staining, employing antibody TKH-2, are sensitive methods for the serological and histologic diagnosis of amniotic fluid embolism (AFE). TKH-2 is a sensitive antibody directed to sialyl Tn (STN), NeuAcalpha2-6GalNAc. Nineteen samples of maternal sera with clinical AFE and 120 control sera and 15 specimen of formalin-fixed, paraffin-embedded lung tissue sections were examined in this study. Tissue sections were stained using the streptavidin-biotin-immunoperoxidase method. The concentration of STN in serum was measured by an immunoradiometric competitive inhibition assay using the monoclonal antibody TKH-2 in a one-step procedure. Remarkable positive TKH-2 stainings were easily seen within the pulmonary vasculature in 14 of the 15 (93%) patients with AFE. The serum STN levels (mean+/-SD) in patients with AFE (110.8+/-48.1 U/ml) showed significantly higher concentrations compared with those of patients with non-AFE (17.3+/-2.6 U/ml) (p <0.01). Seventeen of 19 sera (89%) were diagnosed as AFE by serum TRH-2 level. We conclude that both TKH-2 immunostaining and serum STN assay are sensitive methods to diagnose patients with AFE.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:issn |
0094-6176
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
24
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
479-84
|
pubmed:dateRevised |
2006-3-7
|
pubmed:meshHeading |
pubmed-meshheading:9834017-Antigens, Tumor-Associated, Carbohydrate,
pubmed-meshheading:9834017-Embolism, Amniotic Fluid,
pubmed-meshheading:9834017-Female,
pubmed-meshheading:9834017-Humans,
pubmed-meshheading:9834017-Immunoassay,
pubmed-meshheading:9834017-Pregnancy,
pubmed-meshheading:9834017-Sensitivity and Specificity,
pubmed-meshheading:9834017-Serologic Tests
|
pubmed:year |
1998
|
pubmed:articleTitle |
Serological and immunohistochemical diagnosis of amniotic fluid embolism.
|
pubmed:affiliation |
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Japan.
|
pubmed:publicationType |
Journal Article
|