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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1999-1-22
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pubmed:abstractText |
A new isolating procedure of human hepatocytes has been developed using two-step collagenase digestion by a non-perfusion procedure (NP) of non-wedge liver biopsies. 1. A yield of 2-7 x 10(6) hepatocytes/g liver, 52-95% viability and 13-75% attachment were obtained from liver biopsies weighing 6-60 g, comparable to that obtained when using the classical perfusion procedure (P) to isolate human hepatocytes from wedge liver samples of 50-150 g. 2. In culture, human hepatocytes obtained by NP remained attached to plastic for up to 5 days and displayed the usual morphological characteristics. Their metabolic capacities, assessed by liver-specific albumin and urea synthesis and by CYP-dependent and conjugation pathways, were equivalent to those of human hepatocytes obtained by P. In addition, they responded adequately to specific CYP inducers, demonstrating that they constitute a model in which human drug metabolism and toxicity studies can be performed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0960-3271
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
544-53
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9821017-Adult,
pubmed-meshheading:9821017-Aged,
pubmed-meshheading:9821017-Aged, 80 and over,
pubmed-meshheading:9821017-Albumins,
pubmed-meshheading:9821017-Biopsy,
pubmed-meshheading:9821017-Blotting, Western,
pubmed-meshheading:9821017-Carcinoma, Hepatocellular,
pubmed-meshheading:9821017-Cell Adhesion,
pubmed-meshheading:9821017-Cell Separation,
pubmed-meshheading:9821017-Cell Survival,
pubmed-meshheading:9821017-Collagenases,
pubmed-meshheading:9821017-Cytochrome P-450 Enzyme System,
pubmed-meshheading:9821017-Enzyme Induction,
pubmed-meshheading:9821017-Female,
pubmed-meshheading:9821017-Glutathione,
pubmed-meshheading:9821017-Glutathione Transferase,
pubmed-meshheading:9821017-Humans,
pubmed-meshheading:9821017-Liver Neoplasms,
pubmed-meshheading:9821017-Male,
pubmed-meshheading:9821017-Middle Aged,
pubmed-meshheading:9821017-Tumor Cells, Cultured,
pubmed-meshheading:9821017-Urea
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pubmed:year |
1998
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pubmed:articleTitle |
Metabolic capacities in cultured human hepatocytes obtained by a new isolating procedure from non-wedge small liver biopsies.
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pubmed:affiliation |
Fondation Transplantation, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Comparative Study
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