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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1998-12-7
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pubmed:abstractText |
Tissue mast cell development requires stem cell factor (SCF), whereas helminth-induced intestinal mucosal mast cell hyperplasia also requires T cell-derived factors such as IL-3. We generated progenitor mast cells (PrMC) from mouse bone marrow cells (BMC) in vitro with a triad of SCF, IL-6, and IL-10 that exhibit IL-3-mediated mitogenic and maturation responses. SCF/IL-6/IL-10 transiently elicited a cell subpopulation with the phenotype (c-kit(high)Thy-1(low)) of fetal blood promastocytes at 3 wk of culture that progressed within 1 wk to FcepsilonRI-bearing PrMC, designated PrMCTriad. PrMCTriad lacked mouse mast cell carboxypeptidase A (mMC-CPA) protein, required SCF for IL-3-driven thymidine incorporation, and responded to SCF plus IL-3 with strong mMc-CPA immunoreactivity, clarifying distinct sequential roles for SCF and IL-3 in mast cell development. PrMCTriad, arising from BMC through promastocytes, are metamastocytes that acquire microenvironmentally determined phenotypic features.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
|
pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5143-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9820483-Animals,
pubmed-meshheading:9820483-Bone Marrow Cells,
pubmed-meshheading:9820483-Cell Differentiation,
pubmed-meshheading:9820483-Cell Division,
pubmed-meshheading:9820483-Cell Separation,
pubmed-meshheading:9820483-Cells, Cultured,
pubmed-meshheading:9820483-Drug Synergism,
pubmed-meshheading:9820483-Female,
pubmed-meshheading:9820483-Flow Cytometry,
pubmed-meshheading:9820483-Immunohistochemistry,
pubmed-meshheading:9820483-Interleukin-3,
pubmed-meshheading:9820483-Mast Cells,
pubmed-meshheading:9820483-Mice,
pubmed-meshheading:9820483-Mice, Inbred BALB C,
pubmed-meshheading:9820483-Stem Cell Factor,
pubmed-meshheading:9820483-Stem Cells,
pubmed-meshheading:9820483-Thymidine
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pubmed:year |
1998
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pubmed:articleTitle |
Generation of a novel stem cell factor-dependent mast cell progenitor.
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pubmed:affiliation |
Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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