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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1998-12-31
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|
pubmed:abstractText |
Local production of immunosuppressive cytokines will be one of the most suitable therapeutic strategies against organ-specific autoimmune diabetes. To establish such a new therapy, we constructed recombinant adenoviral vectors with inserted mIL-12p40 (Ad.IL-12p40) and mIL-10 (Ad.IL-10). Sufficient amounts of IL-12p40 and IL-10 were secreted by relevant adenovirus-transfected nonobese diabetic (NOD) islets. Shortly after transfection, 400 NOD islets transfected with Ad.IL-12p40 or Ad.IL-10 were transplanted under the renal capsule of a newly diabetic NOD mouse. NOD mice with IL-12p40-producing islet grafts kept normoglycemia in all of 14 grafted mice for over 4 wk after transplantation. In contrast, NOD mice with IL-10-producing islet grafts became diabetic in all of six grafted mice within 2 wk af-ter transplantation. Reverse transcription-PCR analysis revealed that local production of IL-12p40 led to the decrease of interferon-gamma and the augmentation of transforming growth factor-beta at the graft site. These results suggest that IL-12 plays an important role in the destruction of islet cells at the inflamed site of autoimmunity. Such a local blockade of IL-12 would be a useful gene therapy for human autoimmune diabetes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-1675502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-1727730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-1899142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-1946445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-2205920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-2523712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-2642862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-2663991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-3098614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-3517648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-3999192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-6995140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7485382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7525049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7612223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7624350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7696210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7722337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7761837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7836934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7843232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7860747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7884845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7901851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-7988786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8101546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8103745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8145050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8168635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8181185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8315397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8386517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8476563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8616883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8720612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8799158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8805632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8878437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8893001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8933280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-8976172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-9127018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-9174613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-9218758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-9222642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-9275207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-9341777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9819366-9381527
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9738
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pubmed:author |
pubmed-author:ArisawaKK,
pubmed-author:FujihiraKK,
pubmed-author:HamadaHH,
pubmed-author:KasugaMM,
pubmed-author:MikiMM,
pubmed-author:MoriyamaHH,
pubmed-author:NagataMM,
pubmed-author:OkamotoNN,
pubmed-author:SaitoII,
pubmed-author:YasudaHH,
pubmed-author:YokonoKK,
pubmed-author:YoshidaRR
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1807-14
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9819366-Animals,
pubmed-meshheading:9819366-Mice,
pubmed-meshheading:9819366-Kidney,
pubmed-meshheading:9819366-Insulin,
pubmed-meshheading:9819366-Female,
pubmed-meshheading:9819366-Male,
pubmed-meshheading:9819366-Islets of Langerhans,
pubmed-meshheading:9819366-Islets of Langerhans Transplantation,
pubmed-meshheading:9819366-Adenoviridae,
pubmed-meshheading:9819366-Diabetes Mellitus, Type 1
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