Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1999-2-25
pubmed:abstractText
Enzyme-prodrug therapy for the treatment of cancer is an experimental procedure that is under intensive investigation. However, the relative merits of the various systems for use under specific conditions are still being determined. We have compared the efficacy of cell killing by the herpesvirus thymidine kinase (HSVTK)/ganciclovir and the purine nucleoside phosphorylase (PNP)/9-(beta-M-2-deoxy-erythropentofuranosyl)6-methylpurine enzyme/prodrug systems. These were chosen because of their differential dependence on DNA replication for their mechanism of action. The HSVTK and PNP genes, expressed from the identical prostate-specific antigen promoter, were transduced into human prostate and breast cancers cells using the same human adenovirus vector. The kinetics of cell killing in the presence of the respective prodrugs was monitored using a nondestructive assay that measured total cell bioactivity. The PNP/9-(beta-D-2-deoxy-erythropentofuranosyl)6-methylpurine system was clearly superior in its ability to cause cell death in vitro. Cells were killed in about half the time and at a 5-10-fold lower input of virus relative to the HSVTK/ganciclovir system. The PNP system may offer advantages for the treatment of slow-growing tumors in which the daily proliferative rate is low or in situations in which gene delivery or expression is inefficient.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1078-0432
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2075-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9815599-Antiviral Agents, pubmed-meshheading:9815599-Breast Neoplasms, pubmed-meshheading:9815599-Cell Survival, pubmed-meshheading:9815599-DNA Replication, pubmed-meshheading:9815599-Female, pubmed-meshheading:9815599-Ganciclovir, pubmed-meshheading:9815599-Genetic Vectors, pubmed-meshheading:9815599-Humans, pubmed-meshheading:9815599-Kinetics, pubmed-meshheading:9815599-Male, pubmed-meshheading:9815599-Prodrugs, pubmed-meshheading:9815599-Prostatic Neoplasms, pubmed-meshheading:9815599-Purine-Nucleoside Phosphorylase, pubmed-meshheading:9815599-Purines, pubmed-meshheading:9815599-Recombinant Fusion Proteins, pubmed-meshheading:9815599-Simplexvirus, pubmed-meshheading:9815599-Thymidine Kinase, pubmed-meshheading:9815599-Tumor Cells, Cultured, pubmed-meshheading:9815599-beta-Galactosidase
pubmed:year
1997
pubmed:articleTitle
Relative efficiency of tumor cell killing in vitro by two enzyme-prodrug systems delivered by identical adenovirus vectors.
pubmed:affiliation
Division of Molecular Science, Commonwealth Scientific and Industrial Research Organisation, North Ryde, 2113, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't