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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
47
|
| pubmed:dateCreated |
1998-12-21
|
| pubmed:abstractText |
The pancreas secretes several different lipases. The most abundant is pancreatic triglyceride lipase (PTL). The pancreas also synthesizes two homologues of PTL, the pancreatic lipase-related proteins 1 and 2 (PLRP1 and PLRP2). Cytotoxic T-lymphocytes also express PLRP2 under certain conditions. We sought to determine the role of PLRP2 in fat absorption and in T-cell cytotoxicity by creating a PLRP2-deficient mouse. Adult PLRP2-deficient mice had normal fat absorption. In contrast, suckling PLRP2-deficient mice had fat malabsorption evidenced by increased fecal weight, increased fecal fats, and the presence of undigested and partially digested dietary triglycerides in the feces. As a result, the PLRP2-deficient pups had a decreased rate of weight gain. To assess T cell cytotoxicity, we immunized PLRP2-deficient mice with a mastocytoma cell line, P815, and determined the ability of splenocytes from the immunized mice to kill P815 cells in a 51Cr release assay. PLRP2-deficient cells had deficient killing activity in this assay, and PLRP2-deficient splenocytes released fewer fatty acid from the target cells than did control cells. Our results provide the first evidence of a physiological function for PLRP2. PLRP2 participates in T cell cytotoxicity, and PLRP2 performs a crucial role in the digestion of dietary fats in suckling animals.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
20
|
| pubmed:volume |
273
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
31215-21
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9813028-Age Factors,
pubmed-meshheading:9813028-Animals,
pubmed-meshheading:9813028-Animals, Newborn,
pubmed-meshheading:9813028-Body Constitution,
pubmed-meshheading:9813028-Cytotoxicity, Immunologic,
pubmed-meshheading:9813028-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:9813028-Dietary Fats,
pubmed-meshheading:9813028-Feces,
pubmed-meshheading:9813028-Heterozygote,
pubmed-meshheading:9813028-Intestinal Absorption,
pubmed-meshheading:9813028-Lipase,
pubmed-meshheading:9813028-Mice,
pubmed-meshheading:9813028-Mice, Inbred BALB C,
pubmed-meshheading:9813028-Mice, Inbred DBA,
pubmed-meshheading:9813028-Mice, Mutant Strains,
pubmed-meshheading:9813028-Pancreas,
pubmed-meshheading:9813028-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9813028-Weight Gain
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Decreased neonatal dietary fat absorption and T cell cytotoxicity in pancreatic lipase-related protein 2-deficient mice.
|
| pubmed:affiliation |
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110, USA. Lowe@Kids.wustl.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|