| pubmed-article:9799398 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C0597694 | lld:lifeskim |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C0021547 | lld:lifeskim |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C0596902 | lld:lifeskim |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C1420202 | lld:lifeskim |
| pubmed-article:9799398 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:9799398 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9799398 | pubmed:dateCreated | 1998-12-11 | lld:pubmed |
| pubmed-article:9799398 | pubmed:abstractText | The myo-inositol transporter SMIT is expressed particularly at high extracellular osmolarity and serves to accumulate the osmolyte myo-inositol. Transport of myo-inositol is coupled to the cotransport of Na+ and is electrogenic. In Xenopus oocytes injected with mRNA encoding SMIT but not in water-injected oocytes, myo-inositol creates an inward current that is dependent on the ambient Na+ concentration. The present study has been performed to elucidate the pH dependence of myo-inositol-induced currents. Therefore, Xenopus oocytes were injected with mRNA encoding SMIT and two-electrode voltage-clamp studies were performed. The myo-inositol-induced currents in oocytes expressing SMIT were found to have a sigmoidal dependence on the ambient pH between pH 5.5 and 8.5 with an apparent Ki of 0.21+/-001 microM H+ and a Hill coefficient of 1.80+/-0.16. Kinetic analysis of the myo-inositol-induced currents at pH 8.0 and -90 mV holding potential revealed a Hill coefficient of 0.93+/-0.07 and an apparent Km for myo-inositol of 0.031+/-0.003 mM as well as a Hill coefficient of 1. 64+/-0.24 and an apparent Km of 38.8+/-4.1 mM for Na+. A decrease of the Na+ concentra-tion from 150 mM to 50 mM significantly altered the maximal observed current and increased the apparent Km for myo-inositol. Acidification to pH 6.5 significantly increased the apparent Km for myo-inositol and for Na+ to 0.057+/-0.005 mM and 73. 9+/-4.8 mM, respectively. The Hill coefficients for myo-inositol and Na+ were not affected and remained close to 1 for myo-inositol and 2 for Na+. In summary, acidification impedes SMIT-mediated myo-inositol transport at least partially by decreasing the affinity of the carrier for Na+. The impaired Na+ binding subsequently decreases binding and transport of myo-inositol. | lld:pubmed |
| pubmed-article:9799398 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9799398 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9799398 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9799398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9799398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9799398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9799398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9799398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9799398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9799398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9799398 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9799398 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:9799398 | pubmed:issn | 0031-6768 | lld:pubmed |
| pubmed-article:9799398 | pubmed:author | pubmed-author:LaneDD | lld:pubmed |
| pubmed-article:9799398 | pubmed:author | pubmed-author:HandlerJ SJS | lld:pubmed |
| pubmed-article:9799398 | pubmed:author | pubmed-author:RislerTT | lld:pubmed |
| pubmed-article:9799398 | pubmed:author | pubmed-author:WagnerC ACA | lld:pubmed |
| pubmed-article:9799398 | pubmed:author | pubmed-author:KwonH MHM | lld:pubmed |
| pubmed-article:9799398 | pubmed:author | pubmed-author:BuschA EAE | lld:pubmed |
| pubmed-article:9799398 | pubmed:author | pubmed-author:WaldeggerSS | lld:pubmed |
| pubmed-article:9799398 | pubmed:author | pubmed-author:MatskevitchJJ | lld:pubmed |
| pubmed-article:9799398 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9799398 | pubmed:volume | 436 | lld:pubmed |
| pubmed-article:9799398 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9799398 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9799398 | pubmed:pagination | 854-7 | lld:pubmed |
| pubmed-article:9799398 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9799398 | pubmed:meshHeading | pubmed-meshheading:9799398-... | lld:pubmed |
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| pubmed-article:9799398 | pubmed:meshHeading | pubmed-meshheading:9799398-... | lld:pubmed |
| pubmed-article:9799398 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9799398 | pubmed:articleTitle | Effect of extracellular pH on the myo-inositol transporter SMIT expressed in Xenopus oocytes. | lld:pubmed |
| pubmed-article:9799398 | pubmed:affiliation | Institute for Physiology, University of Tübingen, Gmelinstrasse 5, D-72076 Tübingen, Germany. | lld:pubmed |
| pubmed-article:9799398 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9799398 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9799398 | lld:pubmed |
