Linked Life Data

9797255

Source:http://linkedlifedata.com/resource/pubmed/id/9797255

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1999-1-14
pubmed:abstractText
The purpose of this study was to develop and apply a quantitative optical method suitable for routine measurements of biofilm structures under in situ conditions. A computer program was designed to perform automated investigations of biofilms by using image acquisition and image analysis techniques. To obtain a representative profile of a growing biofilm, a nondestructive procedure was created to study and quantify undisturbed microbial populations within the physical environment of a glass flow cell. Key components of the computer-controlled processing described in this paper are the on-line collection of confocal two-dimensional (2D) cross-sectional images from a preset 3D domain of interest followed by the off-line analysis of these 2D images. With the quantitative extraction of information contained in each image, a three-dimensional reconstruction of the principal biological events can be achieved. The program is convenient to handle and was generated to determine biovolumes and thus facilitate the examination of dynamic processes within biofilms. In the present study, Pseudomonas fluorescens or a green fluorescent protein-expressing Escherichia coli strain, EC12, was inoculated into glass flow cells and the respective monoculture biofilms were analyzed in three dimensions. In this paper we describe a method for the routine measurements of biofilms by using automated image acquisition and semiautomated image analysis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-1610183, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-1917879, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-2200342, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-2408564, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-4397528, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-7535888, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-7574613, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-7592342, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-7685999, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-8357261, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-8596440, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-8707058, http://linkedlifedata.com/resource/pubmed/commentcorrection/9797255-9361441
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4115-27
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Automated confocal laser scanning microscopy and semiautomated image processing for analysis of biofilms.
pubmed:affiliation
Institute of Water Quality Control and Waste Management, Technical University of Munich, D-85748 Garching, Technical University of Munich, D-80290 Munich, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't