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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1999-2-3
|
| pubmed:abstractText |
The vaginal epithelium of normal mice contains lymphocytes of fetal thymic origin that express an invariant Vgamma4/Vdelta1 TCR. The apparent lack of other gammadelta TCR species suggests that a selection mechanism might operate to regulate the localization of gammadelta T cells at this anatomical site. Selection might be connected to the Vgamma4/Vdelta1 TCR or to some homing characteristic of the fetal thymic lineage that appears at day 17-18 of embryonic life. In the present studies, we investigated whether transgenic gammadelta cells expressing a TCR species characteristic of the subpopulation of gammadelta T cells found in the blood, spleen and lymph would translocate to the vaginal epithelium. We found that the transgenic Vgamma2 TCR+ cells did accumulate in the vagina of transgenic mice. Furthermore, like normal vaginal gammadelta T cells, the transgenic vaginal gammadelta T cells expressed the phenotype of recently activated memory/effector T cells (CD44(hi), CD62L-, CD45RB(lo), CD69+). Vaginal gammadelta T cells in normal mice do not express the CD2 and CD28 antigens, but both of these markers are present on transgenic vaginal gammadelta T cells. We observed that a small fraction of splenic transgenic gammadelta T cells had the same surface phenotype as the vaginal transgenic gammadelta T cells, raising the possibility that the gammadelta T cells present in the vaginal epithelium of transgenic mice originated from the peripheral lymphoid organs. Data in support of this possibility came from experiments in which co-incubation of splenic transgenic gammadelta T cells with vaginal epithelial cell suspensions induced the vaginal gammadelta phenotype on the splenic gammadelta T cells. The finding of transgenic gammadelta T cells in the vaginal epithelium suggests that homing of gammadelta T cells to this site is not restricted to gammadelta T cells that express the V4/NS1 invariant TCR. Furthermore, these findings imply that retention of gammadelta T cells in the vaginal epithelium of normal mice is affected by a Vgamma4/Vdelta1-specific mechanism. The finding of a significant level of apoptosis in the transgenic vaginal gammadelta T cells, but not in the normal vaginal gammadelta T cells, could reflect that the mechanism of retention of Vgamma4/Vdelta1 + in the vaginal epithelium involves selective survival at the site.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/CD69 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lymphocyte Homing
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0953-8178
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1509-17
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:9796918-Animals,
pubmed-meshheading:9796918-Antigens, CD,
pubmed-meshheading:9796918-Antigens, CD2,
pubmed-meshheading:9796918-Antigens, CD28,
pubmed-meshheading:9796918-Antigens, CD44,
pubmed-meshheading:9796918-Antigens, CD45,
pubmed-meshheading:9796918-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:9796918-Apoptosis,
pubmed-meshheading:9796918-Biological Markers,
pubmed-meshheading:9796918-Cell Movement,
pubmed-meshheading:9796918-Cells, Cultured,
pubmed-meshheading:9796918-Epithelium,
pubmed-meshheading:9796918-Female,
pubmed-meshheading:9796918-L-Selectin,
pubmed-meshheading:9796918-Lectins, C-Type,
pubmed-meshheading:9796918-Lymphocyte Activation,
pubmed-meshheading:9796918-Mice,
pubmed-meshheading:9796918-Mice, Inbred BALB C,
pubmed-meshheading:9796918-Mice, Transgenic,
pubmed-meshheading:9796918-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:9796918-Receptors, Lymphocyte Homing,
pubmed-meshheading:9796918-Spleen,
pubmed-meshheading:9796918-T-Lymphocytes,
pubmed-meshheading:9796918-Transgenes,
pubmed-meshheading:9796918-Vagina
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Homing of transgenic gammadelta T cells into murine vaginal epithelium.
|
| pubmed:affiliation |
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|