Linked Life Data

9786496

Source:http://linkedlifedata.com/resource/pubmed/id/9786496

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-1-4
pubmed:abstractText
1. The effects of saponin from Ginseng Radix rubra on extracellular matrix metabolism, the activation and synthesis of TGF-beta1, and the modification of TGF-beta receptor in fibroblasts were examined to elucidate the contribution of the TGF-beta pathway to the mechanism of wound healing by saponin. 2. Fibronectin synthesis was analysed by the immunoprecipitation method. Activation and synthesis of TGF-beta1 were measured by ELISA. The expressions of TGF-beta receptors in fibroblasts were examined at protein and mRNA levels by the cross-linking method and Northern blot analysis, respectively. 3. The fibronectin synthesis increased 2.3- and 3.9-fold at fibroblasts treated with 1 and 10 microg ml(-1) of saponin, respectively, compared with that in non-treated cells. Fibronectin synthesis stimulated with 10 microg ml(-1) of saponin was inhibited with 69% by 5 microg ml(-1) of an anti-TGF-beta1 antibody. mRNA of TGF-beta type I receptor increased 4.8- and 4.4-fold at fibroblasts treated with 1 and 10 microg ml(-1) of saponin, respectively, and that of TGF-beta type II receptor also increased 3.4- and 3.2-fold at fibroblasts treated with 1 and 10 microg ml(-1) of saponin, respectively. The significant increases of TGF-beta type I and II receptors and of fibronectin synthesis were observed at the same concentrations of saponin. TGF-beta content increased 1.74- and 1.87-fold at conditioned medium of fibroblasts treated with 100 and 250 microg ml(-1) of saponin, respectively, higher concentrations than those which accelerated fibronectin synthesis. Furthermore, the active TGF-beta content was below 10% of total TGF-beta at each concentration of saponin. 4. These results indicate that saponin stimulates fibronectin synthesis through the changes of TGF-beta receptor expressions in fibroblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-62
pubmed:dateRevised
2008-11-20
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Role of transforming growth factor-beta pathway in the mechanism of wound healing by saponin from Ginseng Radix rubra.
pubmed:affiliation
The Second Department of Internal Medicine, School of Medicine, Chiba University, Chiba City, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't