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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1998-12-11
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pubmed:abstractText |
The nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coli by using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA). The recombinant antigen was reactive with positive human sera, and the reactivity correlated very well (r = 0.9) with that of a whole-virus antigen when tested by EIA with 30 TOS virus-positive and 30 TOS virus-negative serum samples. The results demonstrate that the recombinant N protein can be easily produced in a procaryotic system and used for diagnostic assays for TOS virus immunity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-13571577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-1413515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-1659738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-1714110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-1951851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-7781738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-8096868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-829416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-8460526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-8578009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-8880508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-8991940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-9010288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-9384264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774559-9650974
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3170-2
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9774559-Antibodies, Viral,
pubmed-meshheading:9774559-Antigens, Viral,
pubmed-meshheading:9774559-Base Sequence,
pubmed-meshheading:9774559-Bunyaviridae,
pubmed-meshheading:9774559-Bunyaviridae Infections,
pubmed-meshheading:9774559-DNA Primers,
pubmed-meshheading:9774559-Escherichia coli,
pubmed-meshheading:9774559-Humans,
pubmed-meshheading:9774559-Immunoenzyme Techniques,
pubmed-meshheading:9774559-Immunoglobulin G,
pubmed-meshheading:9774559-Immunoglobulin M,
pubmed-meshheading:9774559-Nucleocapsid Proteins,
pubmed-meshheading:9774559-Recombinant Proteins,
pubmed-meshheading:9774559-Reverse Transcriptase Polymerase Chain Reaction
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pubmed:year |
1998
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pubmed:articleTitle |
Diagnostic potential of Toscana virus N protein expressed in Escherichia coli.
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pubmed:affiliation |
Department of Molecular Biology, Section of Microbiology, University of Siena, 53100 Siena, Italy.
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pubmed:publicationType |
Journal Article
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