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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
43
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pubmed:dateCreated |
1998-11-12
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pubmed:abstractText |
We have examined whether certain secreted factor(s) is involved in oxidized low density lipoprotein (Ox-LDL)-induced murine macrophage growth. An antibody against granulocyte-macrophage colony-stimulating factor (GM-CSF) effectively inhibited Ox-LDL-induced macrophage growth by >80%. Ox-LDL as well as phospholipase A2-treated acetylated LDL enhanced mRNA levels and protein release of GM-CSF from macrophages, while neither acetylated LDL nor lysophosphatidylcholine (lyso-PC) showed such effects. The maximal induction of GM-CSF by Ox-LDL was noted at 4 h, followed by a time-dependent decrease to a basal level within 24 h. Ox-LDL-induced macrophage growth was inhibited by 75% by replacement of the culture medium at 24 h by a fresh medium containing the same concentration of Ox-LDL, when GM-CSF had already returned to the basal level. Thus, a cytokine(s) other than GM-CSF is also expected to participate in Ox-LDL-induced macrophage growth in a later phase. The Ox-LDL-induced GM-CSF release was inhibited by calphostin C, a protein kinase C inhibitor, and was significantly reduced in macrophages from the knockout mice lacking class A, type I and type II macrophage scavenger receptors (MSR-AI/AII). These results taken together indicate that effective endocytosis of lyso-PC of Ox-LDL by macrophages through MSR-AI/AII and subsequent protein kinase C activation have led to GM-CSF release into the medium which may play a priming role in conjunction with other cytokines in Ox-LDL-induced macrophage growth.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Msr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Naphthalenes,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Scavenger,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class A,
http://linkedlifedata.com/resource/pubmed/chemical/calphostin complex,
http://linkedlifedata.com/resource/pubmed/chemical/oxidized low density lipoprotein
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
28305-13
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9774454-Animals,
pubmed-meshheading:9774454-Cell Division,
pubmed-meshheading:9774454-Culture Media, Conditioned,
pubmed-meshheading:9774454-Dose-Response Relationship, Drug,
pubmed-meshheading:9774454-Endocytosis,
pubmed-meshheading:9774454-Enzyme Activation,
pubmed-meshheading:9774454-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9774454-Interleukin-3,
pubmed-meshheading:9774454-Interleukin-5,
pubmed-meshheading:9774454-Lipoproteins, LDL,
pubmed-meshheading:9774454-Lysophosphatidylcholines,
pubmed-meshheading:9774454-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9774454-Macrophages, Peritoneal,
pubmed-meshheading:9774454-Male,
pubmed-meshheading:9774454-Mice,
pubmed-meshheading:9774454-Mice, Knockout,
pubmed-meshheading:9774454-Naphthalenes,
pubmed-meshheading:9774454-Protein Kinase C,
pubmed-meshheading:9774454-Receptors, Immunologic,
pubmed-meshheading:9774454-Receptors, Scavenger,
pubmed-meshheading:9774454-Scavenger Receptors, Class A,
pubmed-meshheading:9774454-Signal Transduction
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pubmed:year |
1998
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pubmed:articleTitle |
Induction of murine macrophage growth by oxidized low density lipoprotein is mediated by granulocyte macrophage colony-stimulating factor.
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pubmed:affiliation |
Department of Biochemistry, Kumamota University School of Medicine, Kumamota 860-0811, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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