| pubmed-article:9771917 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9771917 | lifeskim:mentions | umls-concept:C0020200 | lld:lifeskim |
| pubmed-article:9771917 | lifeskim:mentions | umls-concept:C0140057 | lld:lifeskim |
| pubmed-article:9771917 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9771917 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:9771917 | pubmed:dateCreated | 1998-10-22 | lld:pubmed |
| pubmed-article:9771917 | pubmed:abstractText | In this study we employed the neuroblastoma x glioma NG 108-15 cell line as a model for investigating the effects of long-term activation of cannabinoid receptors on delta opioid receptor desensitization, down-regulation and gene expression. Exposure of NG 108-15 cells to (-)-delta9-tetrahydrocannabinol (delta9-THC) reduced opioid receptor binding, evaluated in intact cells, by approximately 40-45% in cells exposed for 24 h to 50 and 100 nM delta9-THC and by approximately 25% in cells exposed to 10 nM delta9-THC. Lower doses of delta9-THC (0.1 and 1 nM) or a shorter exposure time to the cannabinoid (6 h) were not effective. Down-regulation of 6 opioid receptors was not observed in cells exposed for 24 h to pertussis toxin (PTX) and then treated for 24 h with 100 nM delta9-THC. In cells that were exposed for 24 h to the cannabinoid, the ability of delta9-THC and of the delta opioid receptor agonist [D-Ser2, Leu5, Thr6]enkephalin to inhibit forskolin-stimulated cAMP accumulation was significantly attenuated. Prolonged exposure of NG 108-15 cells to 100 nM delta9-THC produced a significant elevation of steady-state levels of delta opioid receptor mRNA. This effect was not observed in cells pretreated with PTX. The selective cannabinoid receptor antagonist SR 141716A blocked the effects elicited by delta9-THC on delta opioid receptor desensitization, down-regulation and gene expression; thus indicating that these are mediated via activation of cannabinoid receptors. These data demonstrate the existence, in NG 108-15 cells, of a complex cross-talk between the cannabinoid and opioid receptors on prolonged exposure to delta9-THC triggered by changes in signaling through Gi and/or G0-coupled receptors. | lld:pubmed |
| pubmed-article:9771917 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9771917 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9771917 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9771917 | pubmed:issn | 0024-3205 | lld:pubmed |
| pubmed-article:9771917 | pubmed:author | pubmed-author:SpampinatoSS | lld:pubmed |
| pubmed-article:9771917 | pubmed:author | pubmed-author:CampanaGG | lld:pubmed |
| pubmed-article:9771917 | pubmed:author | pubmed-author:Di ToroRR | lld:pubmed |
| pubmed-article:9771917 | pubmed:author | pubmed-author:MurariGG | lld:pubmed |
| pubmed-article:9771917 | pubmed:author | pubmed-author:SciarrettaVV | lld:pubmed |
| pubmed-article:9771917 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9771917 | pubmed:volume | 63 | lld:pubmed |
| pubmed-article:9771917 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9771917 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9771917 | pubmed:pagination | PL197-204 | lld:pubmed |
| pubmed-article:9771917 | pubmed:dateRevised | 2008-8-22 | lld:pubmed |
| pubmed-article:9771917 | pubmed:meshHeading | pubmed-meshheading:9771917-... | lld:pubmed |
| pubmed-article:9771917 | pubmed:meshHeading | pubmed-meshheading:9771917-... | lld:pubmed |
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| pubmed-article:9771917 | pubmed:meshHeading | pubmed-meshheading:9771917-... | lld:pubmed |
| pubmed-article:9771917 | pubmed:meshHeading | pubmed-meshheading:9771917-... | lld:pubmed |
| pubmed-article:9771917 | pubmed:meshHeading | pubmed-meshheading:9771917-... | lld:pubmed |
| pubmed-article:9771917 | pubmed:meshHeading | pubmed-meshheading:9771917-... | lld:pubmed |
| pubmed-article:9771917 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9771917 | pubmed:articleTitle | Regulation of delta opioid receptors by delta9-tetrahydrocannabinol in NG108-15 hybrid cells. | lld:pubmed |
| pubmed-article:9771917 | pubmed:affiliation | Department of Pharmacology, University of Bologna, Italy. | lld:pubmed |
| pubmed-article:9771917 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9771917 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
