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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1998-10-30
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pubmed:abstractText |
The pasABC genes of the proteic plasmid addiction system of broad-host-range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100-fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated tac promoter, they were unable to stabilize a heterologous test plasmid.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-1761233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-1779758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-2120189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-2615761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-2981804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-6390428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-7494469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-8177170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-8262949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765582-9383152
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
180
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5463-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9765582-Antidotes,
pubmed-meshheading:9765582-Bacterial Proteins,
pubmed-meshheading:9765582-Bacterial Toxins,
pubmed-meshheading:9765582-Escherichia coli,
pubmed-meshheading:9765582-Gene Expression Regulation, Bacterial,
pubmed-meshheading:9765582-Genes, Reporter,
pubmed-meshheading:9765582-Plasmids,
pubmed-meshheading:9765582-Promoter Regions, Genetic
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pubmed:year |
1998
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pubmed:articleTitle |
Autoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization.
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pubmed:affiliation |
Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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