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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1998-10-23
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pubmed:abstractText |
Ionotropic, nicotinic receptors have previously been shown to mediate both inhibitory (Cl-dependent) and excitatory (cationic) cholinergic responses in Aplysia neurons. We have used fast perfusion methods of agonist and antagonist application to reevaluate the effects on these receptors of a wide variety of cholinergic compounds, including a number of recently isolated and/or synthesized alpha toxins [alpha-conotoxin (alphaCTx)] from Conus snails. These toxins have been shown in previous studies to discriminate between the many types of nicotinic receptors now known to be expressed in vertebrate muscle, neuroendocrine, and neuronal cells. One of these toxins (alphaCTx ImI from the worm-eating snail Conus imperialis) revealed that two kinetically and pharmacologically distinct elements underlie the ACh-induced Cl-dependent response in Aplysia neurons: one element is a rapidly desensitizing current that is blocked by the toxin; the other is a slowly desensitizing current that is unaffected by the toxin. The two kinetically defined elements were also found to be differentially sensitive to different agonists. Finally, the proportion of the rapidly desensitizing element to the sustained element was found to be cell-specific. These observations led to the conclusion that two distinct nicotinic receptors mediate Cl currents in Aplysia neurons. The receptor mediating the rapidly desensitizing Cl-dependent response shows a strong pharmacological resemblance to the vertebrate alpha-bungarotoxin-sensitive, alpha7-containing receptor, which is permeable to calcium and mediates a rapidly desensitizing excitatory response.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Aconitine,
http://linkedlifedata.com/resource/pubmed/chemical/Alkaloids,
http://linkedlifedata.com/resource/pubmed/chemical/Azocines,
http://linkedlifedata.com/resource/pubmed/chemical/Bungarotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Cations,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Choline,
http://linkedlifedata.com/resource/pubmed/chemical/Cholinergic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Conotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydro-beta-Erythroidine,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Insecticides,
http://linkedlifedata.com/resource/pubmed/chemical/Mollusk Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotinic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotinic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Quinolizines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nicotinic,
http://linkedlifedata.com/resource/pubmed/chemical/Strychnine,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-conotoxin ImI,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-conotoxin MII,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-conotoxin PnIA,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-conotoxin PnIB,
http://linkedlifedata.com/resource/pubmed/chemical/alpha7 nicotinic acetylcholine...,
http://linkedlifedata.com/resource/pubmed/chemical/conotoxin EIVA,
http://linkedlifedata.com/resource/pubmed/chemical/cytisine,
http://linkedlifedata.com/resource/pubmed/chemical/methyllycaconitine,
http://linkedlifedata.com/resource/pubmed/chemical/subecholine
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0270-6474
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8198-213
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pubmed:dateRevised |
2010-6-4
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pubmed:meshHeading |
pubmed-meshheading:9763466-Acetylcholine,
pubmed-meshheading:9763466-Aconitine,
pubmed-meshheading:9763466-Alkaloids,
pubmed-meshheading:9763466-Animals,
pubmed-meshheading:9763466-Aplysia,
pubmed-meshheading:9763466-Azocines,
pubmed-meshheading:9763466-Bungarotoxins,
pubmed-meshheading:9763466-Cations,
pubmed-meshheading:9763466-Chlorides,
pubmed-meshheading:9763466-Choline,
pubmed-meshheading:9763466-Cholinergic Antagonists,
pubmed-meshheading:9763466-Conotoxins,
pubmed-meshheading:9763466-Dihydro-beta-Erythroidine,
pubmed-meshheading:9763466-Electric Conductivity,
pubmed-meshheading:9763466-Glycine Agents,
pubmed-meshheading:9763466-Insecticides,
pubmed-meshheading:9763466-Ion Channel Gating,
pubmed-meshheading:9763466-Mollusk Venoms,
pubmed-meshheading:9763466-Nicotinic Agonists,
pubmed-meshheading:9763466-Nicotinic Antagonists,
pubmed-meshheading:9763466-Oligopeptides,
pubmed-meshheading:9763466-Patch-Clamp Techniques,
pubmed-meshheading:9763466-Peptides,
pubmed-meshheading:9763466-Quinolizines,
pubmed-meshheading:9763466-Receptors, Nicotinic,
pubmed-meshheading:9763466-Strychnine,
pubmed-meshheading:9763466-Vertebrates
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pubmed:year |
1998
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pubmed:articleTitle |
Two distinct nicotinic receptors, one pharmacologically similar to the vertebrate alpha7-containing receptor, mediate Cl currents in aplysia neurons.
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pubmed:affiliation |
Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris 75005, France.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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