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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
40
|
| pubmed:dateCreated |
1998-11-12
|
| pubmed:abstractText |
A high fat diet causes resistance of skeletal muscle glucose transport to insulin and contractions. We tested the hypothesis that fat feeding causes a change in plasma membrane composition that interferes with functioning of glucose transporters and/or insulin receptors. Epitrochlearis muscles of rats fed a high (50% of calories) fat diet for 8 weeks showed approximately 50% decreases in insulin- and contraction-stimulated 3-O-methylglucose transport. Similar decreases in stimulated glucose transport activity occurred in muscles of wild-type mice with 4 weeks of fat feeding. In contrast, GLUT1 overexpressing muscles of transgenic mice fed a high fat diet showed no decreases in their high rates of glucose transport, providing evidence against impaired glucose transporter function. Insulin-stimulated system A amino acid transport, insulin receptor (IR) tyrosine kinase activity, and insulin-stimulated IR and IRS-1 tyrosine phosphorylation were all normal in muscles of rats fed the high fat diet for 8 weeks. However, after 30 weeks on the high fat diet, there was a significant reduction in insulin-stimulated tyrosine phosphorylation in muscle. The increases in GLUT4 at the cell surface induced by insulin or muscle contractions, measured with the 3H-labeled 2-N-4-(1-azi-2,2, 2-trifluoroethyl)-benzoyl-1,3-bis-(D-mannose-4-yloxy)-2-propyla min e photolabel, were 26-36% smaller in muscles of the 8-week high fat-fed rats as compared with control rats. Our findings provide evidence that (a) impairment of muscle glucose transport by 8 weeks of high fat feeding is not due to plasma membrane composition-related reductions in glucose transporter or insulin receptor function, (b) a defect in insulin receptor signaling is a late event, not a primary cause, of the muscle insulin resistance induced by fat feeding, and (c) impaired GLUT4 translocation to the cell surface plays a major role in the decrease in stimulated glucose transport.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-O-Methylglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Dietary Fats,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, rat
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
2
|
| pubmed:volume |
273
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
26157-63
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9748297-3-O-Methylglucose,
pubmed-meshheading:9748297-Amino Acid Transport Systems,
pubmed-meshheading:9748297-Animals,
pubmed-meshheading:9748297-Biological Transport,
pubmed-meshheading:9748297-Blood Glucose,
pubmed-meshheading:9748297-Body Weight,
pubmed-meshheading:9748297-Carrier Proteins,
pubmed-meshheading:9748297-Dietary Fats,
pubmed-meshheading:9748297-Glucose Transporter Type 1,
pubmed-meshheading:9748297-Glucose Transporter Type 4,
pubmed-meshheading:9748297-Insulin,
pubmed-meshheading:9748297-Male,
pubmed-meshheading:9748297-Mice,
pubmed-meshheading:9748297-Mice, Transgenic,
pubmed-meshheading:9748297-Monosaccharide Transport Proteins,
pubmed-meshheading:9748297-Muscle, Skeletal,
pubmed-meshheading:9748297-Muscle Contraction,
pubmed-meshheading:9748297-Muscle Proteins,
pubmed-meshheading:9748297-Protein-Tyrosine Kinases,
pubmed-meshheading:9748297-Rats,
pubmed-meshheading:9748297-Rats, Wistar,
pubmed-meshheading:9748297-Receptor, Insulin
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
A high fat diet impairs stimulation of glucose transport in muscle. Functional evaluation of potential mechanisms.
|
| pubmed:affiliation |
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. phansen@imgate.wustl.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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