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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1998-10-14
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pubmed:abstractText |
In Bacilli, ribosomes or 30 S ribosomal subunits that are stalled or bound on mRNAs can stabilize downstream regions, hence the view that the degradation machinery scans mRNAs from their 5' end. In E. coli, several mRNAs can also be stabilized by secondary structures involving their 5' end. To test whether a bound 30 S subunit can act as a 5' stabilizer in E. coli, we compare here the stabilities of two untranslated variants of the lacZ mRNA, the decay of which is controlled by RNase E. In the first variant, a 35 nt region including the Ribosome Binding Site (RBS) is deleted, whereas in the second it is replaced by an 11 nt-long Shine-Dalgarno (SD) sequence lacking an associated start codon. In the latter variant, an 80 nt fragment encompassing the SD and extending up to the mRNA 5' end was stable in vivo (t1/2>one hour), reflecting 30 S binding. Yet, the full-length message was not more stable than when the SD was absent, although two small decay intermediates retaining the 5' end appear somewhat stabilized. A third variant was constructed in which the RBS is replaced by an insert which can fold back onto the lac leader, creating a putative hairpin involving the mRNA 5' end. The fragment corresponding to this hairpin was stable but, again, the full-length message was not stabilized. Thus, the untranslated lacZ mRNA cannot be protected against RNase E by 5' stabilizers, suggesting that mRNA scanning is not an obligate feature of RNase E-controlled degradation. Altogether, these results suggest important differences in mRNA degradation between E. coli and B. subtilis. In addition, we show that mRNA regions involved in stable hairpins or Shine-Dalgarno pairings can be metabolically stable in E. coli.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-2836
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 1998 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
241-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9735284-Base Sequence,
pubmed-meshheading:9735284-Endoribonucleases,
pubmed-meshheading:9735284-Escherichia coli,
pubmed-meshheading:9735284-Lac Operon,
pubmed-meshheading:9735284-Molecular Sequence Data,
pubmed-meshheading:9735284-Nucleic Acid Conformation,
pubmed-meshheading:9735284-Protein Biosynthesis,
pubmed-meshheading:9735284-RNA, Bacterial,
pubmed-meshheading:9735284-RNA, Messenger,
pubmed-meshheading:9735284-Ribosomes
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pubmed:year |
1998
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pubmed:articleTitle |
In the absence of translation, RNase E can bypass 5' mRNA stabilizers in Escherichia coli.
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pubmed:affiliation |
Laboratoire de Génétique Moléculaire, CNRS URA 1302, Ecole Normale Supérieure, 46 rue d'Ulm, Paris, 75230, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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