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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1998-9-28
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pubmed:abstractText |
Several mutations that cause severe forms of the human disease autosomal dominant retinitis pigmentosa cluster in the C-terminal region of rhodopsin. Recent studies have implicated the C-terminal domain of rhodopsin in its trafficking on specialized post-Golgi membranes to the rod outer segment of the photoreceptor cell. Here we used synthetic peptides as competitive inhibitors of rhodopsin trafficking in the frog retinal cell-free system to delineate the potential regulatory sequence within the C terminus of rhodopsin and model the effects of severe retinitis pigmentosa alleles on rhodopsin sorting. The rhodopsin C-terminal sequence QVS(A)PA is highly conserved among different species. Peptides that correspond to the C terminus of bovine (amino acids 324-348) and frog (amino acids 330-354) rhodopsin inhibited post-Golgi trafficking by 50% and 60%, respectively, and arrested newly synthesized rhodopsin in the trans-Golgi network. Peptides corresponding to the cytoplasmic loops of rhodopsin and other control peptides had no effect. When three naturally occurring mutations: Q344ter (lacking the last five amino acids QVAPA), V345M, and P347S were introduced into the frog C-terminal peptide, the inhibitory activity of the peptides was no longer detectable. These observations suggest that the amino acids QVS(A)PA comprise a signal that is recognized by specific factors in the trans-Golgi network. A lack of recognition of this sequence, because of mutations in the last five amino acids causing autosomal dominant retinitis pigmentosa, most likely results in abnormal post-Golgi membrane formation and in an aberrant subcellular localization of rhodopsin.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-1426275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-1478822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-1544542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-1808803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-1828467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-1833777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-1862076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-1882937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-2021172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-2215617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-2605220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-2780545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-2934469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-6194890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-6342691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7488190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7523628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7552702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7633434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7635666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7643192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7730031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7738098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7806562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-7929568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8106358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8308063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8540855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8567690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8599108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8625403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8643437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8674113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8885257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-8943080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9010208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9050844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9099692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9118953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9151686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9230432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9238089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9296501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9405395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9724753-9527482
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10620-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1998
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pubmed:articleTitle |
Regulation of sorting and post-Golgi trafficking of rhodopsin by its C-terminal sequence QVS(A)PA.
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pubmed:affiliation |
Department of Ophthalmology, University of Michigan, Ann Arbor, MI 48105, USA. dereticd@umich.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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