Linked Life Data

9722540

Source:http://linkedlifedata.com/resource/pubmed/id/9722540

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
36
pubmed:dateCreated
1998-10-15
pubmed:abstractText
Transforming growth factor beta (TGFbeta) superfamily polypeptides regulate cell growth and differentiation by binding to single pass serine/threonine kinases referred to as TGFbeta type I and type II receptors. Signal propagation is dependent upon heteromeric (type I-type II) complex formation and transphosphorylation of the type I receptor by the type II receptor. While many of the phosphorylation events necessary for receptor signaling have recently been characterized, the role of TGFbeta receptor kinase activity in modulating receptor endocytosis has not been addressed. To that end, we have used chimeric receptors consisting of the extracellular domain of the granulocyte/macrophage colony-stimulating factor alpha and beta receptors spliced to the TGFbeta type I and type II transmembrane and cytoplasmic domains to address the specific role of type I and/or type II receptor kinase activity in TGFbeta receptor internalization, down-regulation, and signaling. To inactivate chimeric receptor kinase activity, point mutations in the ATP binding site were made at amino acids 232 and 277 in the type I and type II receptor, respectively. Either of these mutations abolished plasminogen activator inhibitor 1 protein expression stimulated by granulocyte/macrophage colony-stimulating factor activation of chimeric heteromeric type I-type II TGFbeta receptors. They did not, however, modulate TGFbeta signaling stimulated through the endogenous TGFbeta receptor. Although TGFbeta receptor signaling was dependent upon the kinase activity of both chimeric receptors, the initial endocytic response was distinctly regulated by type I and/or type II receptor kinase activity. For instance, while heteromeric receptor complexes containing a kinase-inactive type I receptor were endocytosed similarly to wild type complexes, the kinase activity of the type II TGFbeta receptor was necessary for optimal internalization and receptor down-regulation. Furthermore, these responses were shown to occur independently of type II receptor autophosphorylation but require a type II receptor capable of transphosphorylation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23118-25
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9722540-Activin Receptors, Type I, pubmed-meshheading:9722540-Animals, pubmed-meshheading:9722540-Biological Transport, pubmed-meshheading:9722540-Clathrin, pubmed-meshheading:9722540-Clone Cells, pubmed-meshheading:9722540-Down-Regulation, pubmed-meshheading:9722540-Endocytosis, pubmed-meshheading:9722540-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:9722540-Ligands, pubmed-meshheading:9722540-Mesoderm, pubmed-meshheading:9722540-Mice, pubmed-meshheading:9722540-Models, Biological, pubmed-meshheading:9722540-Mutagenesis, Site-Directed, pubmed-meshheading:9722540-Phosphorylation, pubmed-meshheading:9722540-Plasminogen Activator Inhibitor 1, pubmed-meshheading:9722540-Protein-Serine-Threonine Kinases, pubmed-meshheading:9722540-Receptors, Transforming Growth Factor beta, pubmed-meshheading:9722540-Recombinant Fusion Proteins, pubmed-meshheading:9722540-Signal Transduction
pubmed:year
1998
pubmed:articleTitle
Differential requirement for type I and type II transforming growth factor beta receptor kinase activity in ligand-mediated receptor endocytosis.
pubmed:affiliation
Thoracic Research Unit and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.