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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8 Pt 1
|
| pubmed:dateCreated |
1998-8-20
|
| pubmed:abstractText |
Quantitation of lysozyme in human milk was performed by a microparticle-enhanced nephelometric immunoassay based on the measurement of the light scattered during the competitive immunoagglutination of a microparticle-lysozyme conjugate with an anti-lysozyme antiserum. This immunoassay has a detection limit of 8 microg/L of reaction mixture and can be performed using diluted milk (1:6000, in reaction mixture), excluding sample pretreatment. Human milk lysozyme can be quantified over the concentration range 0.09-1.50 g/L, with within- and between-run coefficients of variation <5%. Changes in the lysozyme concentration of human milk during lactation were determined in 636 samples. Lysozyme concentrations (mean +/- SE) decreased from colostrum (0.36 +/- 0.02 g/L) to transitional milk (0.30 +/- 0.01 g/L) and mature milk during days 15-42 (0.30 +/- 0.01 g/L), then increased in the mature milk during days 43-56 (0.35 +/- 0.01 g/L) and especially during days 57-84 (0.83 +/- 0.05 g/L). The proportion of lysozyme contributing to total protein was found to rise during lactation and was as follows: colostrum (1.7%), transitional milk (2.3%), and mature milk from days 15-28 (2.7%), days 29-42 (3.1%), days 43-56 (3.8%), and days 57-84 (7.3%). The assay developed for milk was also suitable for the determination of lysozyme in other human body fluids.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0009-9147
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1610-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9702946-Adolescent,
pubmed-meshheading:9702946-Adult,
pubmed-meshheading:9702946-Body Fluids,
pubmed-meshheading:9702946-Female,
pubmed-meshheading:9702946-Humans,
pubmed-meshheading:9702946-Immunoassay,
pubmed-meshheading:9702946-Lactation,
pubmed-meshheading:9702946-Microspheres,
pubmed-meshheading:9702946-Middle Aged,
pubmed-meshheading:9702946-Milk, Human,
pubmed-meshheading:9702946-Muramidase,
pubmed-meshheading:9702946-Nephelometry and Turbidimetry,
pubmed-meshheading:9702946-Saliva,
pubmed-meshheading:9702946-Time Factors
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Microparticle-enhanced nephelometric immunoassay of lysozyme in milk and other human body fluids.
|
| pubmed:affiliation |
GRIP, Immunology Laboratory, Faculty of Medicine, Vandoeuvre les Nancy, France. paulmont@grip.u-nancy.fr
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|