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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-9-24
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pubmed:abstractText |
The natural resistance-associated macrophage protein 1 (NRAMP1) was mapped in the pig for study as a potential candidate gene in controlling pig resistance to Salmonella infection. Primers were designed from the pig cDNA to amplify a 1.6 kb fragment between exons 1 and 3. By using a pig-rodent somatic cell hybrid panel, NRAMP1 was mapped to pig chromosome 15 (SSC15) with 100% probability, and the regional assignment was SSC15q23-26 with 87% concordance. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) marker was developed by using the HinfI enzyme and three alleles were identified from a population including 11 breeds. Linkage analysis confirmed the physical assignment by using the PiGMaP reference families. Pig NRAMP1 was linked to SSC15 markers S0088, S0149 and S0284 (LOD > 3). A small population study revealed large allele frequency differences among tested breeds. An A allele is only observed in dam (white) lines whereas a similar exclusivity of the C allele was seen in sire (colored) breeds.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0268-9146
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
138-40
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9699275-Alleles,
pubmed-meshheading:9699275-Animals,
pubmed-meshheading:9699275-Animals, Domestic,
pubmed-meshheading:9699275-Animals, Wild,
pubmed-meshheading:9699275-Carrier Proteins,
pubmed-meshheading:9699275-Cation Transport Proteins,
pubmed-meshheading:9699275-Chromosome Mapping,
pubmed-meshheading:9699275-DNA, Complementary,
pubmed-meshheading:9699275-DNA Primers,
pubmed-meshheading:9699275-Genetic Markers,
pubmed-meshheading:9699275-Genetic Variation,
pubmed-meshheading:9699275-Hybrid Cells,
pubmed-meshheading:9699275-Immunity, Innate,
pubmed-meshheading:9699275-Macrophages,
pubmed-meshheading:9699275-Membrane Proteins,
pubmed-meshheading:9699275-Polymerase Chain Reaction,
pubmed-meshheading:9699275-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:9699275-Population Dynamics,
pubmed-meshheading:9699275-Restriction Mapping,
pubmed-meshheading:9699275-Rodentia,
pubmed-meshheading:9699275-Salmonella Infections, Animal,
pubmed-meshheading:9699275-Swine,
pubmed-meshheading:9699275-Swine Diseases
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pubmed:year |
1998
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pubmed:articleTitle |
Mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) gene to pig chromosome 15.
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pubmed:affiliation |
Department of Animal Science, Iowa State University, Ames 50011, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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