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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1998-9-1
|
| pubmed:databankReference | |
| pubmed:abstractText |
Three full length 1-aminocyclopropane-1-carboxylate (ACC) oxidase cDNA clones (pNG-ACO1, 1,254 bp; pNG-ACO2, 1,198 bp; and pNG-ACO3, 1,053 bp) were isolated from the TMV-treated leaf cDNA library of Nicotiana glutinosa plant. They share a high degree of sequence identity (78-81%) throughout the coding regions but are divergent within the 3'-untranslated regions. The gene-specific probes were prepared using these regions to investigate the differential expression of the ACC oxidase gene family in various organs and in response to a multitude of biotic and abiotic stresses in N. glutinosa plants. All three genes were transcriptionally active displaying unique patterns of expression. Both the pNG-ACO1 and pNG-ACO3 transcripts highly accumulated during the senescence of leaves, while the pNG-ACO2 mRNA was constitutively present. In addition, the NG-ACO1 and NG-ACO3 transcripts were predominantly found in roots whereas the NG-ACO2 mRNA was mainly in stems. Upon TMV infection, both NG-ACO1 and NG-ACO3 were markedly induced, but in mock treatment which has an effect of mild wounding, only the NG-ACO3 gene was induced. Furthermore, salicylic acid and CuSO4 treatments of leaves increased the level of NG-ACO1 and NG-ACO3 transcripts, while they did not affect the NG-ACO2 gene expression. Results showed that both the NG-ACO1 and NG-ACO3 genes were highly inducible by ethylene and methyl jasmonate treatments, with NG-ACO3 being more responsive. By contrast, NG-ACO2 did not respond to these growth regulators. Thus, it appears that there are two groups of ACC oxidase transcripts expressed in leaf tissue of N. glutinosa, either stress-induced or constitutive. The possible molecular mechanism of differential regulation of ACC oxidase gene expression and its physiological significance are discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0032-0781
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
39
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
565-73
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9697341-Amino Acid Oxidoreductases,
pubmed-meshheading:9697341-Amino Acid Sequence,
pubmed-meshheading:9697341-Base Sequence,
pubmed-meshheading:9697341-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9697341-Gene Expression Regulation, Plant,
pubmed-meshheading:9697341-Gene Library,
pubmed-meshheading:9697341-Genes, Plant,
pubmed-meshheading:9697341-Genetic Variation,
pubmed-meshheading:9697341-Molecular Sequence Data,
pubmed-meshheading:9697341-Multigene Family,
pubmed-meshheading:9697341-Plant Leaves,
pubmed-meshheading:9697341-Plant Roots,
pubmed-meshheading:9697341-Plants, Toxic,
pubmed-meshheading:9697341-RNA, Messenger,
pubmed-meshheading:9697341-Restriction Mapping,
pubmed-meshheading:9697341-Sequence Alignment,
pubmed-meshheading:9697341-Sequence Homology, Amino Acid,
pubmed-meshheading:9697341-Tobacco,
pubmed-meshheading:9697341-Transcription, Genetic
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Biotic and abiotic stress-related expression of 1-aminocyclopropane-1-carboxylate oxidase gene family in Nicotiana glutinosa L.
|
| pubmed:affiliation |
Department of Biology, College of Science, Yonsei University, Seoul, Korea.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|