Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
1998-8-6
pubmed:abstractText
The unusual temperature effect on the retention of 17 alpha-estradiol, 17 beta-estradiol and equilin in reversed-phase liquid chromatography has been observed. Capacity factors (k') of the steroids were measured using mobile phase with different concentrations of beta-cyclodextrin (from 0 to 16 mM) in a fixed mobile phase composition (acetonitrile-water) and wide range of column temperatures (from 5 degrees to 80 degrees C). The plots of capacity factors vs reciprocal of absolute temperature are nonlinear in each case. At subambient temperatures the capacity factors decreased with temperature decrease. This effect is more evident for the natural 17 beta-estradiol than for its 17 alpha-isomer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
543-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Effect of temperature on separation of estradiol stereoisomers and equilin by liquid chromatography using mobile phases modified with beta-cyclodextrin.
pubmed:affiliation
Medical Academy, Faculty of Pharmacy, Gda?sk, Poland.
pubmed:publicationType
Journal Article