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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1 Pt 1
|
| pubmed:dateCreated |
1998-8-25
|
| pubmed:abstractText |
Recently, marked oxygen dependence of respiration by isolated mitochondria after exposure to prolonged hypoxia has been described. Because mitochondrial oxygen-dependent respiration could significantly influence oxygen consumption during critical illness, we sought to confirm the oxygen-dependent behavior of mitochondria. We hypothesized that mitochondria isolated during sepsis would exhibit increased oxygen dependence. We isolated rat liver mitochondria 16 h after cecal ligation and puncture and found a 30-40% greater oxygen uptake compared with control rats under state 3 conditions. Mitochondria incubated in deoxygenated buffer were studied for oxygen dependence at 10-min intervals for 90 min. Mitochondrial respiration after reoxygenation was stable over a 60-min period of hypoxia for control rats and decreased slightly for septic rats (10-15%). State 3 respiration was 10% lower when mitochondria were reoxygenated at low (15-25 Torr) versus high (90-100 Torr) and low (10-15 Torr) versus intermediate (40-45 Torr) oxygen tension. Oxygen consumption with ascorbate+N, N, N', N'-tetramethyl-p-phenylenediamine was 20% lower at low versus high oxygen tension. No increase in oxygen dependence was observed during 1 h of hypoxic incubation. Our data indicate only a modest oxygen dependence of respiration between 10 and 100 Torr, which is similar for septic and control mitochondria. Additionally, oxygen dependence did not increase significantly during a 1-h hypoxic exposure for well-coupled mitochondrial preparations.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
275
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
L139-44
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9688945-Animals,
pubmed-meshheading:9688945-Anoxia,
pubmed-meshheading:9688945-Ascorbic Acid,
pubmed-meshheading:9688945-Cecum,
pubmed-meshheading:9688945-Kinetics,
pubmed-meshheading:9688945-Male,
pubmed-meshheading:9688945-Mitochondria, Liver,
pubmed-meshheading:9688945-Oxygen Consumption,
pubmed-meshheading:9688945-Rats,
pubmed-meshheading:9688945-Rats, Sprague-Dawley,
pubmed-meshheading:9688945-Reference Values,
pubmed-meshheading:9688945-Sepsis,
pubmed-meshheading:9688945-Tetramethylphenylenediamine,
pubmed-meshheading:9688945-Time Factors
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Mitochondrial respiration after sepsis and prolonged hypoxia.
|
| pubmed:affiliation |
Department of Medicine, University of Texas-Houston Medical Center, Houston, Texas 77030, USA.
|
| pubmed:publicationType |
Journal Article
|