Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-10-16
pubmed:abstractText
Relatively little is known about the properties of the NMDA receptor in avian species. In the present study, NMDA receptor pharmacology and function has been studied in the embryonic chick brain. The competitive antagonist ligand [3H]CGP 39653 bound to telencephali membranes from 17-day-old chick embryos with KD and Bmax values of 6.6 nM and 3.3 pmol/mg protein, respectively. The binding was inhibited by CGS 19755, L-glutamate, R-CPP and D-AP5 with pI50 values of 7.57, 7.49, 7.28 and 7.08, respectively. L-glycine, S-AMPA, kainate and MK-801 only weakly affected the binding. The inhibitory potency of NMDA (pI50 value 6.98), however, was greater than seen in other species, suggesting a species difference in receptor structure. Such a possibility was investigated by use of two NMDA-R1 antibodies, AB 1516 and 69921 A, which recognize the C-terminal region and an intracellular domain of the rat receptor, respectively. Immunoreactive signals (approximately 116 kDa) were found for a rat brain homogenate, whereas only 69921A gave an immunoreactive signal in the chick. The functional status of the NMDA receptors was investigated in serum-free cultures of neurons from 8-day-old chick embryos. Glutamate and NMDA were found concentration-dependently, and in a manner that could be antagonized by CGS 19755 and MK-801, to produce cell death. Thus, the present study indicates that chick embryonic brain expresses functional NMDA receptors that are pharmacologically and possibly structurally different from their rodent counterparts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0028-1298
pubmed:author
pubmed:issnType
Print
pubmed:volume
357
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
625-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9686938-2-Amino-5-phosphonovalerate, pubmed-meshheading:9686938-Animals, pubmed-meshheading:9686938-Autoradiography, pubmed-meshheading:9686938-Blotting, Western, pubmed-meshheading:9686938-Brain, pubmed-meshheading:9686938-Brain Chemistry, pubmed-meshheading:9686938-Calcium, pubmed-meshheading:9686938-Cell Count, pubmed-meshheading:9686938-Cells, Cultured, pubmed-meshheading:9686938-Chick Embryo, pubmed-meshheading:9686938-Dizocilpine Maleate, pubmed-meshheading:9686938-Excitatory Amino Acid Antagonists, pubmed-meshheading:9686938-Excitatory Amino Acids, pubmed-meshheading:9686938-Glutamic Acid, pubmed-meshheading:9686938-Immunohistochemistry, pubmed-meshheading:9686938-Kainic Acid, pubmed-meshheading:9686938-Neurons, pubmed-meshheading:9686938-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:9686938-Telencephalon
pubmed:year
1998
pubmed:articleTitle
Characterization of the N-methyl-D-aspartate (NMDA) receptor in the embryonic chick brain.
pubmed:affiliation
Department of Pharmacology, Umeå University, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't