Linked Life Data

9682483

Source:http://linkedlifedata.com/resource/pubmed/id/9682483

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-9-17
pubmed:databankReference
pubmed:abstractText
The nucleotide sequence of the alpha-L-arabinofuranosidase gene arfB from Clostridium stercorarium was determined. The deduced protein has a molecular mass of 56.2 kDa with an amino terminus identical to the N-terminal sequence of the purified mature enzyme from C. stercorarium. Its sequence is homologous to arabinofuranosidases of glycosyl hydrolase family 51. Sequence alignment and cluster analysis reveal three new members of glycosyl hydrolase family 51, allowing for the definition of highly conserved regions. Two of these regions are remarkably similar to the most conserved regions within several other families of glycosyl hydrolases, which have in common a (beta/alpha)8-barrel as the core super-secondary structure, and allow to predict the acid/base catalyst and the nucleophile of the active site.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
337-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Nucleotide sequence of arfB of Clostridium stercorarium, and prediction of catalytic residues of alpha-L-arabinofuranosidases based on local similarity with several families of glycosyl hydrolases.
pubmed:affiliation
Institute of Molecular Genetics, Russian Academy of Science, Moscow, Russia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't