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rdf:type |
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lifeskim:mentions |
umls-concept:C0003316,
umls-concept:C0008109,
umls-concept:C0026336,
umls-concept:C0029341,
umls-concept:C0033809,
umls-concept:C0205191,
umls-concept:C0591833,
umls-concept:C0668201,
umls-concept:C0805586,
umls-concept:C0876973,
umls-concept:C1545588
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pubmed:issue |
8
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pubmed:dateCreated |
1998-8-20
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pubmed:abstractText |
The ability of a chimeric influenza virus containing, within the antigenic B site of its hemagglutinin, an 11-amino-acid (AEGRAINRRVE) insert from the peptide 10 epitope of outer membrane (OM) protein F of Pseudomonas aeruginosa to serve as a protective vaccine against P. aeruginosa was tested by using the murine chronic pulmonary infection model. Mice immunized with the chimeric virus developed antibodies that reacted in an enzyme-linked immunosorbent assay with peptide 10, with purified protein F, and with whole cells of various immunotype strains of P. aeruginosa but failed to react with a protein F-deficient strain of P. aeruginosa. The chimeric-virus antisera reacted specifically with protein F alone when immunoblotted against proteins extracted from cell envelopes of each of the seven Fisher-Devlin immunotype strains and had significantly greater in vitro opsonic activity for P. aeruginosa than did antisera from wild-type influenza virus-immunized mice. Subsequent to intratracheal challenge with agar-encased cells of P. aeruginosa, chimeric-virus-immunized mice developed significantly fewer severe lung lesions than did control mice immunized with the wild-type influenza virus. Furthermore, the chimeric influenza virus-immunized group had a significantly smaller percentage of mice with >5 x 10(3) CFU of P. aeruginosa in their lungs upon bacterial quantitation than did the control group. These data indicate that chimeric influenza viruses expressing epitopes of OM protein F warrant continued development as vaccines to prevent pulmonary infections caused by P. aeruginosa.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-1370088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-1379985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-2536688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-2833440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-3033095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-3115901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-3133390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-3431954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-561860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-6183370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-6296139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-6315589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-6323316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7474077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7525709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7539410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7580798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7622889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7685119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7692083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7697234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-7775991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-8106759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-8429543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-8645828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-8701588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-8717398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-8876139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9673294-9382753
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3990-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9673294-Antibodies, Bacterial,
pubmed-meshheading:9673294-Antigens, Bacterial,
pubmed-meshheading:9673294-Bacterial Vaccines,
pubmed-meshheading:9673294-Disease Models, Animal,
pubmed-meshheading:9673294-Epitopes, B-Lymphocyte,
pubmed-meshheading:9673294-Genetic Vectors,
pubmed-meshheading:9673294-Humans,
pubmed-meshheading:9673294-Influenza A virus,
pubmed-meshheading:9673294-Lung Diseases,
pubmed-meshheading:9673294-Porins,
pubmed-meshheading:9673294-Pseudomonas Infections,
pubmed-meshheading:9673294-Vaccines, Synthetic
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pubmed:year |
1998
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pubmed:articleTitle |
A chimeric influenza virus expressing an epitope of outer membrane protein F of Pseudomonas aeruginosa affords protection against challenge with P. aeruginosa in a murine model of chronic pulmonary infection.
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pubmed:affiliation |
Department of Microbiology and Immunology, Louisiana State University Medical Center, School of Medicine in Shreveport, Shreveport, Louisiana 71130-3932, USA. Jstacz@lsumc.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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