Linked Life Data

9666730

Source:http://linkedlifedata.com/resource/pubmed/id/9666730

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1998-9-15
pubmed:abstractText
To allow rat brain lactate measurement in vivo, a specific sensor based on a carbon fiber (phi = 30 microns) microelectrode coated with lactate oxidase was prepared. Combined with the differential normal pulse voltammetry measurement method, such a sensor, with a sensitivity of 9.15 +/- 0.91 mA.M-1.cm-2, provided a lactate linear response in concentrations ranging from 0.1 to 2.0 mM. The measurements performed appeared to be essentially insensitive to usual interference caused by the electroactive compounds present in the brain (ascorbic acid and peptides). In vivo detection performed in the cortex of the anesthetized rat led to the determination of a lactate concentration of 0.41 +/- 0.02 mM. Moreover, to validate the results obtained in vivo, an ex vivo determination of the lactate level was also performed in samples of brain tissue, plasma, and cerebrospinal fluid, using both voltammetry and a clinical analyzer with colorimetric-based detection. A good correlation was observed between the sets of data established by both methods.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2618-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
In vivo voltammetric detection of rat brain lactate with carbon fiber microelectrodes coated with lactate oxidase.
pubmed:affiliation
Laboratory of Physicochemistry of Interfaces, UMR CNRS 5621 IFoS, Ecole Centrale de Lyon, Ecully, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't