Linked Life Data

9649632

Source:http://linkedlifedata.com/resource/pubmed/id/9649632

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1998-8-17
pubmed:abstractText
The Serial Analysis of Gene Expression (SAGE) method, described in 1995 by Velculescu et al ., represents a powerful means to compare gene expression between two mRNA populations. An improvement to SAGE that removes contaminating linker molecules, which compromise the efficiency of the method, has been developed. This modification utilises biotinylated PCR primers, which generate biotinylated linkers at an early stage in the SAGE protocol, thus allowing removal of the unwanted linkers by binding to streptavidin-coated magnetic beads at a later stage. The application of this modification resulted in the rapid generation of high ditag yields and clones with large average insert sizes.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3445-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Enhanced concatemer cloning-a modification to the SAGE (Serial Analysis of Gene Expression) technique.
pubmed:affiliation
The Richard Dimbleby Department of Cancer Research, I.C.R.F. Laboratory, Rayne Institute, 4th Floor Lambeth Wing, St Thomas's Hospital, Lambeth Palace Road, London SE1 7EH, UK. powell@icrf.icnet.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't