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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1998-7-14
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pubmed:abstractText |
The 4.6-kb double-stranded (ds) RNA of Trichomonas vaginalis virus (TVV)-T1 has been shown to encode two overlapping genes, cap and pol. In this study, a serum for specifically detecting viral cap gene product was raised against a recombinant protein, and sera for specifically detecting pol gene product were raised against synthetic oligopeptides. A 75-kDa major protein and a 160-kDa minor protein were detected by anti-CAP serum in a TVV-T1 sample, indicating that the 75-kDa protein is the viral capsid protein. The 160-kDa protein alone was also detected by two distinct anti-POL sera, indicating that the pol gene is expressed as a CAP-POL fusion protein. These results suggest that the TVV-T1 genome is arranged into a cap-pol organization in a manner similar to that of viruses in family Totiviridae.
|
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0304-8608
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
963-70
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9645201-Animals,
pubmed-meshheading:9645201-Antibodies, Viral,
pubmed-meshheading:9645201-Capsid,
pubmed-meshheading:9645201-Escherichia coli,
pubmed-meshheading:9645201-Gene Products, pol,
pubmed-meshheading:9645201-Genes, Viral,
pubmed-meshheading:9645201-Genome, Viral,
pubmed-meshheading:9645201-Humans,
pubmed-meshheading:9645201-RNA Viruses,
pubmed-meshheading:9645201-Recombinant Proteins,
pubmed-meshheading:9645201-Totiviridae,
pubmed-meshheading:9645201-Trichomonas vaginalis,
pubmed-meshheading:9645201-Viral Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
Characterization of Trichomonas vaginalis virus proteins in the pathogenic protozoan T. vaginalis.
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pubmed:affiliation |
Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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