Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-7-10
pubmed:abstractText
Rubella virus E1 glycoprotein normally complexes with E2 in the endoplasmic reticulum (ER) to form a heterodimer that is transported to and retained in the Golgi complex. In a previous study, we showed that in the absence of E2, unassembled E1 subunits accumulate in a tubular pre-Golgi compartment whose morphology and biochemical properties are distinct from both rough ER and Golgi. We hypothesized that this compartment corresponds to hypertrophied ER exit sites that have expanded in response to overexpression of E1. In the present study we constructed BHK cells stably expressing E1 protein containing a cytoplasmically disposed epitope and isolated the pre-Golgi compartment from these cells by cell fractionation and immunoisolation. Double label indirect immunofluorescence in cells and immunoblotting of immunoisolated tubular networks revealed that proteins involved in formation of ER-derived transport vesicles, namely p58/ERGIC 53, Sec23p, and Sec13p, were concentrated in the E1-containing pre-Golgi compartment. Furthermore, budding structures were evident in these membrane profiles, and a highly abundant but unknown 65-kDa protein was also present. By comparison, marker proteins of the rough ER, Golgi, and COPI vesicles were not enriched in these membranes. These results demonstrate that the composition of the tubular networks corresponds to that expected of ER exit sites. Accordingly, we propose the name SEREC (smooth ER exit compartment) for this structure.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-1096303, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-1421566, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-1500424, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-1808196, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-1924322, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-2477140, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-2688707, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-2722778, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-2845137, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-3058161, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-3182932, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-3316245, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-3536963, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-3649277, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-4307201, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-6498939, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-7615693, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-7749196, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-7865879, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-7876089, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-7876304, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-7991558, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8004676, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8027184, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8314846, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8314869, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8314870, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8408057, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8451644, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8511398, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8522600, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8548805, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8858160, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8922375, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-8972206, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-9148925, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-9151666, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-9261052, http://linkedlifedata.com/resource/pubmed/commentcorrection/9614173-9323141
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1059-1524
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1265-78
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9614173-Animals, pubmed-meshheading:9614173-Binding Sites, pubmed-meshheading:9614173-COP-Coated Vesicles, pubmed-meshheading:9614173-Cell Line, pubmed-meshheading:9614173-Coated Vesicles, pubmed-meshheading:9614173-Cricetinae, pubmed-meshheading:9614173-Endoplasmic Reticulum, Rough, pubmed-meshheading:9614173-Fungal Proteins, pubmed-meshheading:9614173-GTPase-Activating Proteins, pubmed-meshheading:9614173-Golgi Apparatus, pubmed-meshheading:9614173-Intracellular Membranes, pubmed-meshheading:9614173-Mannose-Binding Lectins, pubmed-meshheading:9614173-Membrane Proteins, pubmed-meshheading:9614173-Mutagenesis, pubmed-meshheading:9614173-Nuclear Pore Complex Proteins, pubmed-meshheading:9614173-Saccharomyces cerevisiae Proteins, pubmed-meshheading:9614173-Transfection, pubmed-meshheading:9614173-Viral Envelope Proteins
pubmed:year
1998
pubmed:articleTitle
Immunoisolation and characterization of a subdomain of the endoplasmic reticulum that concentrates proteins involved in COPII vesicle biogenesis.
pubmed:affiliation
Department of Cell Biology and Anatomy, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
pubmed:publicationType
Journal Article
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